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| Title | Structural insights into higher-order natural RNA-only multimers. |
|---|---|
| Journal, issue, pages | Nat Struct Mol Biol, Vol. 32, Issue 10, Page 2012-2021, Year 2025 |
| Publish date | Aug 6, 2025 |
 Authors | Shengchun Zhang / Ran Yi / Linfeng An / Ji Liu / Xuebiao Yao / Shanshan Li / Kaiming Zhang /  |
| PubMed Abstract | RNA-only complexes adopt intricate three-dimensional structures to fulfill diverse functions independently of protein partners. Although multimeric RNA-only structures have been engineered in ...RNA-only complexes adopt intricate three-dimensional structures to fulfill diverse functions independently of protein partners. Although multimeric RNA-only structures have been engineered in synthetic RNA nanomaterials, naturally occurring RNA-only complexes have primarily been observed as monomers or dimers, leaving higher-order assemblies largely unexplored. ROOL (rumen-originating, ornate, large) and GOLLD (giant, ornate, lake- and Lactobacillales-derived) RNAs are conserved non-coding RNAs with complex secondary structures, but their high-resolution architectures remain unknown. Here, we determine the cryo-electron microscopy structures of UCC118-Rool RNA, Sag-Golld RNA and Env38-Golld RNA at 1.96-2.98 Å resolution, revealing their distinct hexameric, decameric and tetradecameric assemblies. These higher-order architectures are stabilized by an array of tertiary motifs such as kissing loops and tetraloop-receptor motifs, underscoring the conserved principles of RNA self-assembly. By elucidating the molecular details of these higher-order RNA-only assemblies, this study expands our understanding of RNA-based architectures and broadens the scope of RNA structural biology. |
 External links | Nat Struct Mol Biol / PubMed:40770114 |
| Methods | EM (single particle) |
| Resolution | 1.96 - 3.83 Å |
| Structure data | EMDB-63014, PDB-9le3: EMDB-63015, PDB-9le5: EMDB-63016, PDB-9le6:  EMDB-63019: Consensus map of Sag-18RS21 Golld RNA EMDB-63022, PDB-9lec: EMDB-63023, PDB-9lee:  EMDB-63026: Consensus map of Sag-18RS21-tRNASer Golld RNA EMDB-63028, PDB-9lel: EMDB-63029, PDB-9lem:  EMDB-63099: Consensus map of Env38-Golld RNA EMDB-63100, PDB-9lhk: EMDB-63101, PDB-9lhl: EMDB-63678, PDB-9m78: EMDB-63679, PDB-9m79: |
| Source |
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 Keywords | RNA / Natural RNA nanocages; large RNA; cryo-EM; Rool / Natural RNA nanocages; large RNA; cryo-EM; Golld / Natural RNA nanocages; large RNA; cryo-EM; Golld RNA / Natural RNA-Only Multimer / cryo-EM / Oligomer / Rool. |
ligilactobacillus salivarius ucc118 (bacteria)
uncultured caudovirales phage (virus)