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- EMDB-63023: Composite map of Sag-18RS21 Golld RNA -

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Basic information

Entry
Database: EMDB / ID: EMD-63023
TitleComposite map of Sag-18RS21 Golld RNA
Map dataComposite map of Sag-18RS21 Golld RNA
Sample
  • Complex: Composite map of Sag-18RS21 Golld RNA
    • RNA: Sag-18RS21 Golld RNA
KeywordsNatural RNA nanocages / large RNA / cryo-EM / Golld / RNA
Biological speciesStreptococcus agalactiae 18RS21 (bacteria)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.51 Å
AuthorsZhang K / Li S
Funding support China, 1 items
OrganizationGrant numberCountry
National Natural Science Foundation of China (NSFC) China
CitationJournal: Nat Struct Mol Biol / Year: 2025
Title: Structural insights into higher-order natural RNA-only multimers.
Authors: Shengchun Zhang / Ran Yi / Linfeng An / Ji Liu / Xuebiao Yao / Shanshan Li / Kaiming Zhang /
Abstract: RNA-only complexes adopt intricate three-dimensional structures to fulfill diverse functions independently of protein partners. Although multimeric RNA-only structures have been engineered in ...RNA-only complexes adopt intricate three-dimensional structures to fulfill diverse functions independently of protein partners. Although multimeric RNA-only structures have been engineered in synthetic RNA nanomaterials, naturally occurring RNA-only complexes have primarily been observed as monomers or dimers, leaving higher-order assemblies largely unexplored. ROOL (rumen-originating, ornate, large) and GOLLD (giant, ornate, lake- and Lactobacillales-derived) RNAs are conserved non-coding RNAs with complex secondary structures, but their high-resolution architectures remain unknown. Here, we determine the cryo-electron microscopy structures of UCC118-Rool RNA, Sag-Golld RNA and Env38-Golld RNA at 1.96-2.98 Å resolution, revealing their distinct hexameric, decameric and tetradecameric assemblies. These higher-order architectures are stabilized by an array of tertiary motifs such as kissing loops and tetraloop-receptor motifs, underscoring the conserved principles of RNA self-assembly. By elucidating the molecular details of these higher-order RNA-only assemblies, this study expands our understanding of RNA-based architectures and broadens the scope of RNA structural biology.
History
DepositionJan 7, 2025-
Header (metadata) releaseSep 10, 2025-
Map releaseSep 10, 2025-
UpdateOct 29, 2025-
Current statusOct 29, 2025Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_63023.png

Downloads & links

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Map

FileDownload / File: emd_63023.map.gz / Format: CCP4 / Size: 512 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map of Sag-18RS21 Golld RNA
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesX (Sec.)Y (Row.)Z (Col.)
0.86 Å/pix.
x 512 pix.
= 440.32 Å		0.86 Å/pix.
x 512 pix.
= 440.32 Å		0.86 Å/pix.
x 512 pix.
= 440.32 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.86 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 8.0
Minimum - Maximum-37.930602999999998 - 97.315939999999998
Average (Standard dev.)0.029489344 (±1.8478065)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderZYX
Origin000
Dimensions512512512
Spacing512512512
CellA=B=C: 440.32 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Composite map of Sag-18RS21 Golld RNA

EntireName: Composite map of Sag-18RS21 Golld RNA
Components
  • Complex: Composite map of Sag-18RS21 Golld RNA
    • RNA: Sag-18RS21 Golld RNA

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Supramolecule #1: Composite map of Sag-18RS21 Golld RNA

SupramoleculeName: Composite map of Sag-18RS21 Golld RNA / type: complex / ID: 1 / Parent: 0 / Macromolecule list: all
Source (natural)Organism: Streptococcus agalactiae 18RS21 (bacteria)
Molecular weightTheoretical: 1.2 MDa

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Macromolecule #1: Sag-18RS21 Golld RNA

MacromoleculeName: Sag-18RS21 Golld RNA / type: rna / ID: 1 / Number of copies: 10
Source (natural)Organism: Streptococcus agalactiae 18RS21 (bacteria)
Molecular weightTheoretical: 122.22018 KDa
SequenceString: GGAGUAGGCG UUGCGCAUUU UGUUGCUCAA AAGGCGACGA AACGCAAGGC AAUGCACGUC UGCGAUACAC GAAAACAAUG CUAUUUGUU GAAAAUAUUG GAAUAAAGCA AAAGUCAUUG CCCGUCGCAA ACGAAAGUGU GCUUCGGUAG CUAGGCUACC U GCUAGAGU ...String:
GGAGUAGGCG UUGCGCAUUU UGUUGCUCAA AAGGCGACGA AACGCAAGGC AAUGCACGUC UGCGAUACAC GAAAACAAUG CUAUUUGUU GAAAAUAUUG GAAUAAAGCA AAAGUCAUUG CCCGUCGCAA ACGAAAGUGU GCUUCGGUAG CUAGGCUACC U GCUAGAGU CUCGCAAGGA UAAUAGCAAA GUCAAAGAGU AAAGCAGCUU AGACCUUUAG CGGGGUUUUC GUUAAUUGAA AA AUGGCUU AGUAGUUUGC GGCGUAACGA GUGGUUAGCG AUACUAACCG CGCAUGGUUG UUACUUGAAG GGAUUUGAGU GGA UAAAAA ACUAAAACAU AAGGUUUUGA AAGACAACUG ACUAAACGUG UAAUCUCAGC GU

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 46.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Nominal defocus max: 1.5 µm / Nominal defocus min: 0.7000000000000001 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: NONE
Startup modelType of model: NONE
Final reconstructionApplied symmetry - Point group: D5 (2x5 fold dihedral) / Resolution.type: BY AUTHOR / Resolution: 2.51 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 3571910
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD

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