EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of cooperative strigolactone perception by the MAX2 ubiquitin ligase-receptor-substrate complex.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 10291, Year 2025
掲載日	2025年11月21日
著者	Alexandra I Vancea / Brandon Huntington / Wieland Steinchen / Christos G Savva / Umar F Shahul Hameed / Stefan T Arold /
PubMed 要旨	Strigolactones are plant hormones that regulate development and mediate interactions with soil organisms, including the germination of parasitic plants such as Striga hermonthica. Strigolactone ...Strigolactones are plant hormones that regulate development and mediate interactions with soil organisms, including the germination of parasitic plants such as Striga hermonthica. Strigolactone perception by receptors initiates the degradation of transcriptional repressors via E3 ubiquitin ligases, but the mechanistic link between hormone binding and substrate ubiquitination has remained unclear. We determine cryogenic electron microscopy structures of the receptor-ligase-substrate complex, composed of Arabidopsis ASK1 and substrate, and Striga F-box and receptor proteins. Strigolactone hydrolysis by the receptor, which covalently retains the D-ring, is a prerequisite for complex formation. The substrate engages the complex through two domains, forming a dynamic interface that stabilises the receptor-ligase assembly and repositions the ASK1, suggesting a mechanism for efficient ubiquitination. Here, we show how dynamic, multivalent interactions within the receptor-ligase-substrate complex translate hormone perception into targeted protein degradation, providing insight into how plants integrate hormonal signals into developmental decisions.
リンク	Nat Commun / PubMed:41271672 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 4.3 Å
構造データ	62397 9kkx EMDB-62397, PDB-9kkx: Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 1) with covalently bound D-ring 手法: EM (単粒子) / 解像度: 2.8 Å 62401 9kld EMDB-62401, PDB-9kld: Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 2) with covalently bound D-ring 手法: EM (単粒子) / 解像度: 2.9 Å 62407 9klk EMDB-62407, PDB-9klk: Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 4) with covalently bound D-ring 手法: EM (単粒子) / 解像度: 2.9 Å 62408 9kll EMDB-62408, PDB-9kll: Striga MAX2-ASK1 complex 手法: EM (単粒子) / 解像度: 2.7 Å EMDB-62414: Striga MAX2-ASK1 2:2 dimer 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-62415: Rice D3-ASK1 complex in presence of ShHTL7 with GR24 手法: EM (単粒子) / 解像度: 4.3 Å 62417 9klv EMDB-62417, PDB-9klv: Strigolactone-induced ASK1-MAX2-HTL7-SMAX1 complex (Class 3) with covalently bound D-ring 手法: EM (単粒子) / 解像度: 3.3 Å
化合物	ChemComp-GR2: (3E,3aR,8bS)-3-({[(2R)-4-methyl-5-oxo-2,5-dihydrofuran-2-yl]oxy}methylidene)-3,3a,4,8b-tetrahydro-2H-indeno[1,2-b]furan-2-one / (+)-GR-24
由来	arabidopsis thaliana (シロイヌナズナ) striga hermonthica (植物) Oryza sativa (イネ)
キーワード	SIGNALING PROTEIN / Strigolactone / SCF / Ubiquitination