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- EMDB-62414: Striga MAX2-ASK1 2:2 dimer -

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ID or keywords:

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Basic information

Entry
Database: EMDB / ID: EMD-62414
TitleStriga MAX2-ASK1 2:2 dimer
Map dataUnsharpened map of the 2:2 ASK1-MAX2 dimer
Sample
  • Complex: Strigolactone signalling complex of ASK1-MAX2-HTL-SMAX1 induced by GR24
    • Complex: ASK1
      • Protein or peptide: A. thaliana ASK1
    • Complex: MAX2
      • Protein or peptide: S. hermonthica MAX2
KeywordsStrigolactone / SCF / Ubiquitination / SIGNALING PROTEIN
Function / homology
Function and homology information


phragmoplast / jasmonic acid mediated signaling pathway / ethylene-activated signaling pathway / response to jasmonic acid / response to auxin / auxin-activated signaling pathway / negative regulation of DNA recombination / SCF ubiquitin ligase complex / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / chromosome segregation ...phragmoplast / jasmonic acid mediated signaling pathway / ethylene-activated signaling pathway / response to jasmonic acid / response to auxin / auxin-activated signaling pathway / negative regulation of DNA recombination / SCF ubiquitin ligase complex / SCF-dependent proteasomal ubiquitin-dependent protein catabolic process / chromosome segregation / microtubule cytoskeleton organization / spindle / ubiquitin-dependent protein catabolic process / protein ubiquitination / mitochondrion / nucleus / cytosol
Similarity search - Function
COI1, F-box / F-box / Leucine-rich repeat, cysteine-containing subtype / Leucine-rich repeat - CC (cysteine-containing) subfamily / SKP1 component, dimerisation / S-phase kinase-associated protein 1 / SKP1-like, dimerisation domain superfamily / Skp1 family, dimerisation domain / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain ...COI1, F-box / F-box / Leucine-rich repeat, cysteine-containing subtype / Leucine-rich repeat - CC (cysteine-containing) subfamily / SKP1 component, dimerisation / S-phase kinase-associated protein 1 / SKP1-like, dimerisation domain superfamily / Skp1 family, dimerisation domain / S-phase kinase-associated protein 1-like / SKP1 component, POZ domain / Skp1 family, tetramerisation domain / Found in Skp1 protein family / SKP1/BTB/POZ domain superfamily / Leucine-rich repeat domain superfamily
Similarity search - Domain/homology
SKP1-like protein 1A / F-box protein
Similarity search - Component
Biological speciesArabidopsis thaliana (thale cress) / Striga hermonthica (purple witchweed)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsVancea AI / Huntington B / Savva CG / Arold ST
Funding support Saudi Arabia, 1 items
OrganizationGrant numberCountry
Other privateURF/1/4039-01-01 & URF/1/4080-01-01 Saudi Arabia
CitationJournal: To Be Published
Title: Mechanism of cooperative strigolactone perception by the MAX2 ubiquitin ligase-receptor-substrate complex
Authors: Vancea AI / Huntington B / Steinchen W / Savva CG / Hameed UFS / Arold ST
History
DepositionNov 14, 2024-
Header (metadata) releaseSep 17, 2025-
Map releaseSep 17, 2025-
UpdateOct 1, 2025-
Current statusOct 1, 2025Processing site: PDBj / Status: Released

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Structure visualization

Supplemental images

emd_62414.png

Downloads & links

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Map

FileDownload / File: emd_62414.map.gz / Format: CCP4 / Size: 178 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationUnsharpened map of the 2:2 ASK1-MAX2 dimer
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.73 Å/pix.
x 360 pix.
= 262.8 Å		0.73 Å/pix.
x 360 pix.
= 262.8 Å		0.73 Å/pix.
x 360 pix.
= 262.8 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.73 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.00126
Minimum - Maximum-0.005882329 - 0.013276033
Average (Standard dev.)0.00000869695 (±0.0003075065)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions360360360
Spacing360360360
CellA=B=C: 262.80002 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_62414_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #2

Fileemd_62414_half_map_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: #1

Fileemd_62414_half_map_2.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : Strigolactone signalling complex of ASK1-MAX2-HTL-SMAX1 induced b...

EntireName: Strigolactone signalling complex of ASK1-MAX2-HTL-SMAX1 induced by GR24
Components
  • Complex: Strigolactone signalling complex of ASK1-MAX2-HTL-SMAX1 induced by GR24
    • Complex: ASK1
      • Protein or peptide: A. thaliana ASK1
    • Complex: MAX2
      • Protein or peptide: S. hermonthica MAX2

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Supramolecule #1: Strigolactone signalling complex of ASK1-MAX2-HTL-SMAX1 induced b...

SupramoleculeName: Strigolactone signalling complex of ASK1-MAX2-HTL-SMAX1 induced by GR24
type: complex / ID: 1 / Parent: 0 / Macromolecule list: all

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Supramolecule #2: ASK1

SupramoleculeName: ASK1 / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #2
Source (natural)Organism: Arabidopsis thaliana (thale cress)

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Supramolecule #3: MAX2

SupramoleculeName: MAX2 / type: complex / ID: 3 / Parent: 1 / Macromolecule list: #1
Source (natural)Organism: Striga hermonthica (purple witchweed)

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Macromolecule #1: S. hermonthica MAX2

MacromoleculeName: S. hermonthica MAX2 / type: protein_or_peptide / ID: 1 / Enantiomer: LEVO
Source (natural)Organism: Striga hermonthica (purple witchweed)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString: MHHHHHHHHH SSGWSHPQFE KGGGSGGGSL EVLFQGPLTP KTNYIPFPSP IHQTPFQTDL APMAAAAAAA TTALNDLPDV ILSNIMAGVS DVRSRNSASL VCHKWYLLER ATRSALTLRG NIRDLFMLPT CFQSTSHLDL SLISPWGHPL TSAADPDSAL IGHLLRHAFP ...String:
MHHHHHHHHH SSGWSHPQFE KGGGSGGGSL EVLFQGPLTP KTNYIPFPSP IHQTPFQTDL APMAAAAAAA TTALNDLPDV ILSNIMAGVS DVRSRNSASL VCHKWYLLER ATRSALTLRG NIRDLFMLPT CFQSTSHLDL SLISPWGHPL TSAADPDSAL IGHLLRHAFP SVTSLAIYAR DPSTIHIVVP QWPDLERLKL VRWHQRPQTD AAGDELKLLI SECGTLKSLD LSSFYCWTDD VPAALGSCPT FAANLKSLNL LNSSFSEGFK SDEIKAITKA CPNLREFRAS CMFDPRYIGH AGDEALVSIS VNCPKLEILH LADTNALSSA RSDFDPDERE GLGQEEAKIN AATLIEVFSG LPLLEELALD LCNNVRDSGP ALEVLNSKCP KLKSVKLGQF HGISLPVESK LDGIALCQGL ESLSIRNVDD LTDMGLIAIG RGCYRLAKFE VYGCKKITVR GMRTMASLLR KTLVDVKIAA CKKLGAVQSL KALEPIQDRV ERLHIDCDWD CPDEEEPSDY FDSAAEYNEC DDEMYSVRKR ARYTYDLNSS SSGLDVNVEG YDEDKTWARL RYVSLWIFVG QLLTPLVAAG LNDCPELEEI SIKVEGDCRV LSRPTVREFG LTTLLNYPKL SRMHLDCGDI NGYAHTAPSG QMDLSLWERF YLIGVGHLGL TELNYWPPQD RDVNQRSLSL PAAGLLQECN RLRKLFIHGT AHEHFMMFFL RIEGLRDVQL RADYYPAPEN DMSTEMRADS CSRFEVALNR RQISDSSGSS G

UniProtKB: F-box protein

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Macromolecule #2: A. thaliana ASK1

MacromoleculeName: A. thaliana ASK1 / type: protein_or_peptide / ID: 2 / Enantiomer: LEVO
Source (natural)Organism: Arabidopsis thaliana (thale cress)
Recombinant expressionOrganism: Spodoptera frugiperda (fall armyworm)
SequenceString:
MSAKKIVLKS SDGESFEVEE AVALESQTIA HMVEDDCVDN GVPLPNVTSK ILAKVIEYCK RHVEAAASKA EAVEGAATSD DDLKAWDADF MKIDQATLFE LILAANYLNI KNLLDLTCQT VADMIKGKTP EEIRTTFNIK NDFTPEEEEE VRRENQWAFE

UniProtKB: SKP1-like protein 1A

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

Concentration0.35 mg/mL
BufferpH: 7.5
Component:
ConcentrationFormulaName
20.0 mMC8H18N2O4SHEPES
200.0 mMNaClsodium chloride
0.5 mMC9H15O6PTCEP
GridModel: UltrAuFoil R1.2/1.3 / Material: GOLD / Mesh: 300 / Support film - Material: GOLD / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE / Pretreatment - Time: 30 sec. / Pretreatment - Atmosphere: AIR / Details: 30 mA
VitrificationCryogen name: ETHANE / Chamber humidity: 100 % / Chamber temperature: 277 K / Instrument: FEI VITROBOT MARK IV

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Electron microscopy

MicroscopeTFS KRIOS
TemperatureMin: 80.0 K / Max: 90.0 K
Specialist opticsEnergy filter - Name: TFS Selectris X / Energy filter - Slit width: 10 eV
Image recordingFilm or detector model: TFS FALCON 4i (4k x 4k) / Number grids imaged: 1 / Number real images: 20175 / Average exposure time: 3.0 sec. / Average electron dose: 44.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsC2 aperture diameter: 50.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 2.7 µm / Nominal defocus min: 1.5 µm / Nominal magnification: 165000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Startup modelType of model: INSILICO MODEL
Final reconstructionApplied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Details: Anisotropic / Number images used: 309078
Initial angle assignmentType: MAXIMUM LIKELIHOOD
Final angle assignmentType: MAXIMUM LIKELIHOOD
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial modelChain - Source name: AlphaFold / Chain - Initial model type: in silico model
RefinementSpace: REAL / Protocol: FLEXIBLE FIT

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