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Structure paper

TitleStructure-guided engineering of a polyphosphate kinase 2 class III from an Erysipelotrichaceae bacterium to produce base-modified purine nucleotides.
Journal, issue, pagesRsc Chem Biol, Vol. 6, Page 1328-1335, Year 2025
Publish dateFeb 20, 2025 (structure data deposition date)
AuthorsMitton-Fry, R.M. / Rasche, R. / Lawrence-Dorner, A.M. / Eschenbach, J. / Tekath, A. / Rentmeister, A. / Kummel, D. / Cornelissen, N.V.
External linksRsc Chem Biol / PubMed:40667417
MethodsX-ray diffraction
Resolution1.7 - 2.311 Å
Structure data

PDB-9igq:
Crystal structure of PPK2 class III from Erysipelotrichaceae bacterium in complex with AppCH2p and polyphosphate
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-9igr:
Crystal structure of PPK2 class III from Erysipelotrichaceae bacterium in complex with polyphosphate
Method: X-RAY DIFFRACTION / Resolution: 2.311 Å

Chemicals

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-6YY:
bis[oxidanyl-[oxidanyl-[oxidanyl-[oxidanyl(phosphonooxy)phosphoryl]oxy-phosphoryl]oxy-phosphoryl]oxy-phosphoryl] hydrogen phosphate

ChemComp-GOL:
GLYCEROL

ChemComp-BEZ:
BENZOIC ACID

ChemComp-MG:
Unknown entry

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

PDB-1i4d:
CRYSTAL STRUCTURE ANALYSIS OF RAC1-GDP COMPLEXED WITH ARFAPTIN (P21)

ChemComp-HOH:
WATER

ChemComp-PO4:
PHOSPHATE ION

Source
  • erysipelotrichaceae bacterium (bacteria)
KeywordsTRANSFERASE / Polyphosphate Kinase / PPK2-III / NTP / EbPPK / NTP Transferase

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