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Structure paper

TitleCyanobacterial Argonautes and Cas4 family nucleases cooperate to interfere with invading DNA.
Journal, issue, pagesMol. Cell, Vol. 85, Page 1920-, Year 2025
Publish dateFeb 9, 2025 (structure data deposition date)
AuthorsBobadilla Ugarte, P. / Halter, S. / Mutte, S.K. / Heijstek, C. / Niault, T. / Terenin, I. / Barendse, P. / Koopal, B. / Roosjen, M. / Boeren, S. ...Bobadilla Ugarte, P. / Halter, S. / Mutte, S.K. / Heijstek, C. / Niault, T. / Terenin, I. / Barendse, P. / Koopal, B. / Roosjen, M. / Boeren, S. / Hauryliuk, V. / Jinek, M. / Westphal, A.H. / Swarts, D.C.
External linksMol. Cell / PubMed:40288374
MethodsX-ray diffraction
Resolution1.57 - 2.52 Å
Structure data

PDB-9iab:
Structure of the Argonaute-associated Cas4 family protein 1 (ACE1) from Chroococcidiopsis thermalis (CtACE1)
Method: X-RAY DIFFRACTION / Resolution: 1.57 Å

PDB-9iac:
Structure of the Argonaute-associated Cas4 family protein 1 (ACE1) from Microcystis aeruginosa (MaACE1)
Method: X-RAY DIFFRACTION / Resolution: 2.52 Å

PDB-9iad:
Chroococcidiopsis thermalis Argonaute (CtAgo) bound to the 3' end of a guide DNA
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

Source
  • chroococcidiopsis thermalis (bacteria)
  • microcystis aeruginosa (bacteria)
  • synthetic construct (others)
KeywordsIMMUNE SYSTEM / Cas4 family protein / deoxyribonuclease / hydrolase / Prokaryotic Argonaute / slicer

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