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Structure paper

TitleRecruitment of bifunctional regulator thermospermine to methylated ribosomes directs xylem fate.
Journal, issue, pagesScience, Vol. 391, Issue 6786, Page 694-699, Year 2026
Publish dateFeb 12, 2026
AuthorsDonghwi Ko / Raili Ruonala / Alexandre Faille / Eva Hellmann / Hanna Help / Huili Liu / Ronni Nielsen / Anders Haakonsson / Nuria De Diego / Anja Paatero / Mariia V Shcherbii / Karolina Stefanowicz / Sanja Ćavar Zeljković / Tine Drud Lundager Rasmussen / Ondrej Novak / Zsuzsanna Bodi / Gugan Eswaran / Brecht Wybouw / Matthieu Bourdon / Cristina Urbez / Xiaonan Liu / Kari Salokas / Tiina Öhman / Tanya Waldie / Petri Törönen / Sedeer El-Showk / Martin Balcerowicz / Fabrice Besnard / Xiaomin Liu / Patrick Perkins / Serina Mazzoni-Putman / Julia P Vainonen / Maija Sierla / Mikko J Frilander / Susanne Mandrup / Teva Vernoux / Karin Ljung / Alejandro Ferrando / Miguel A Blazquez / Liisa Holm / Rupert Fray / Markku Varjosalo / Ottoline Leyser / Ville O Paavilainen / Ari Pekka Mähönen / Anna Stepanova / Jose Alonso / Steffen Heber / Robert Malinowski / Finn Kirpekar / Alan J Warren / Ykä Helariutta /
PubMed AbstractPolyamines are often associated with ribosomes and are thought to stabilize their integrity. In , the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR- ...Polyamines are often associated with ribosomes and are thought to stabilize their integrity. In , the polyamine thermospermine (tSpm) affects xylem cell fate. tSpm induces translation of SUPPRESSOR-OF-ACAULIS51 (SAC51) and SAC51-LIKEs (SACLs), which inhibit heterodimerization of the xylem development proteins LONESOME-HIGHWAY (LHW) and TARGET-OF-MONOPTEROS5. Here, we report a methyltransferase, OVERACHIEVER, that methylates the peptidyl transferase center of the 25 ribosomal RNA (rRNA). Residue mU2952 promotes functional tSpm binding to a specific site connecting the P-site transfer RNA (tRNA) with rRNA residues in the peptidyl transferase center. This interaction enhances the translation of SACLs but inhibits that of LHW. Our study uncovers the dependency between a conserved rRNA base methylation and a polyamine in orchestrating cell fate decisions, highlighting a role for the ribosome chemical landscape in translational regulation.
External linksScience / PubMed:41678627
MethodsEM (single particle)
Resolution1.82 - 2.25 Å
Structure data

51820

9h3g

EMDB-51820, PDB-9h3g:
Structure of the Arabidopsis thaliana 80S ribosome in complex with P- and E-site tRNAs and mRNA
Method: EM (single particle) / Resolution: 1.82 Å

51899

9h6i

EMDB-51899, PDB-9h6i:
Structure of the Arabidopsis thaliana 80S ribosome in complex with P- and E-site tRNAs, mRNA, and thermospermine
Method: EM (single particle) / Resolution: 2.2 Å

52095

9hes

EMDB-52095, PDB-9hes:
Structure of the Arabidopsis thaliana 80S ribosome OVAC mutant in complex with P- and E-site tRNAs, mRNA, and thermospermine
Method: EM (single particle) / Resolution: 2.25 Å

52299

9hmw

EMDB-52299, PDB-9hmw:
Structure of the Arabidopsis thaliana 80S ribosome OVAC mutant in complex with P- and E-site tRNAs and mRNA
Method: EM (single particle) / Resolution: 2.25 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-TER:
N-(3-AMINO-PROPYL)-N-(5-AMINOPROPYL)-1,4-DIAMINOBUTANE

ChemComp-SPD:
SPERMIDINE

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

Source
  • arabidopsis thaliana (thale cress)
KeywordsRIBOSOME / plant / thermospermine / methylation / rRNA modification / thaliana / polyamines / ovac / 3-methyluridine

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