EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Myeloperoxidase transforms chromatin into neutrophil extracellular traps.
ジャーナル・号・ページ	Nature, Vol. 647, Issue 8090, Page 747-756, Year 2025
掲載日	2025年9月17日
著者	Garth Lawrence Burn / Tobias Raisch / Sebastian Tacke / Moritz Winkler / Daniel Prumbaum / Stephanie Thee / Niclas Gimber / Stefan Raunser / Arturo Zychlinsky /
PubMed 要旨	Neutrophils, the most abundant and biotoxic immune cells, extrude nuclear DNA into the extracellular space to maintain homeostasis. Termed neutrophil extracellular traps (NETs), these protein- ...Neutrophils, the most abundant and biotoxic immune cells, extrude nuclear DNA into the extracellular space to maintain homeostasis. Termed neutrophil extracellular traps (NETs), these protein-modified and decondensed extracellular DNA scaffolds control infection and are involved in coagulation, autoimmunity and cancer. Here we show how myeloperoxidase (MPO), a highly expressed neutrophil protein, disassembles nucleosomes, thereby facilitating NET formation, yet also binds stably to NETs extracellularly. We describe how the oligomeric status of MPO governs both outcomes. MPO dimers interact with nucleosomal DNA using one protomer and concurrently dock into the nucleosome acidic patch with the other protomer. As a consequence, dimeric MPO displaces DNA from the core complex, culminating in nucleosome disassembly. On the other hand, MPO monomers stably interact with the nucleosome acidic patch without making concomitant DNA contacts, explaining how monomeric MPO binds to and licences NETs to confer hypohalous acid production in the extracellular space. Our data demonstrate that the binding of MPO to chromatin is governed by specific molecular interactions that transform chromatin into a non-replicative, non-encoding state that offers new biological functions in a cell-free manner. We propose that MPO is, to our knowledge, the first member of a class of proteins that convert chromatin into an immune effector.
リンク	Nature / PubMed:40963017 / PubMed Central
手法	EM (単粒子)
解像度	2.79 - 3.99 Å
構造データ	51295 9gen EMDB-51295, PDB-9gen: Recombinant Myeloperoxidase bound to nucleosome core particle 手法: EM (単粒子) / 解像度: 3.76 Å 51296 9geo EMDB-51296, PDB-9geo: Nucleosome core particle 手法: EM (単粒子) / 解像度: 2.79 Å 51297 9gep EMDB-51297, PDB-9gep: Native monomeric Myeloperoxidase bound to nucleosome core particle 手法: EM (単粒子) / 解像度: 2.89 Å EMDB-51298: Native dimeric Myeloperoxidase bound to nucleosome core particle; nucleosome focused map 手法: EM (単粒子) / 解像度: 3.01 Å EMDB-51299: Native dimeric Myeloperoxidase bound to nucleosome core particle; MPO focused map 手法: EM (単粒子) / 解像度: 2.98 Å EMDB-51300: Native dimeric Myeloperoxidase bound to nucleosome core particle; consensus map 手法: EM (単粒子) / 解像度: 3.12 Å 51301 9geq EMDB-51301, PDB-9geq: Native dimeric Myeloperoxidase bound to nucleosome core particle; composite map 手法: EM (単粒子) / 解像度: 3.12 Å EMDB-51302: Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state, nucleosome focused map 手法: EM (単粒子) / 解像度: 3.52 Å EMDB-51303: Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state, map focused on MPO 手法: EM (単粒子) / 解像度: 3.87 Å EMDB-51304: Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state, consensus map 手法: EM (単粒子) / 解像度: 3.58 Å 51305 9ger EMDB-51305, PDB-9ger: Native dimeric Myeloperoxidase bound to nucleosome core particle, intermediate state; composite map 手法: EM (単粒子) / 解像度: 3.58 Å EMDB-51306: Native monomeric Myeloperoxidase bound to nucleosome core particle, late time point 手法: EM (単粒子) / 解像度: 3.99 Å 52865 9ihd EMDB-52865, PDB-9ihd: Nucleosome core particle bound by one molecule of DTT-reduced native monomeric myeloperoxidase 手法: EM (単粒子) / 解像度: 2.97 Å 52866 9ihe EMDB-52866, PDB-9ihe: Nucleosome core particle bound by two molecules of DTT-reduced native monomeric myeloperoxidase 手法: EM (単粒子) / 解像度: 2.95 Å EMDB-52867: Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase; map focused on nucleosome/MPO monomer 手法: EM (単粒子) / 解像度: 3.13 Å EMDB-52868: Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase; map focused on MPO dimer 手法: EM (単粒子) / 解像度: 3.17 Å EMDB-52869: Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase; consensus map 手法: EM (単粒子) / 解像度: 3.16 Å 52870 9ihf EMDB-52870, PDB-9ihf: Nucleosome core particle bound by one monomer and one dimer of of DTT-reduced native myeloperoxidase 手法: EM (単粒子) / 解像度: 3.16 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-HOH: WATER ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-CL: Unknown entry / クロリド ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	homo sapiens (ヒト) xenopus laevis (アフリカツメガエル) synthetic construct (人工物)
キーワード	IMMUNE SYSTEM / Peroxidase / innate immunity / histone / acidic patch / NUCLEAR PROTEIN / NETs