EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanistic basis of temperature adaptation in microtubule dynamics across frog species.
ジャーナル・号・ページ	Curr Biol, Vol. 35, Issue 3, Page 612-628.e6, Year 2025
掲載日	2025年2月3日
著者	Luca Troman / Ella de Gaulejac / Abin Biswas / Jennifer Stiens / Benno Kuropka / Carolyn A Moores / Simone Reber /
PubMed 要旨	Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a ...Cellular processes are remarkably effective across diverse temperature ranges, even with highly conserved proteins. In the context of the microtubule cytoskeleton, which is critically involved in a wide range of cellular activities, this is particularly striking, as tubulin is one of the most conserved proteins while microtubule dynamic instability is highly temperature sensitive. Here, we leverage the diversity of natural tubulin variants from three closely related frog species that live at different temperatures. We determine the microtubule structure across all three species at between 3.0 and 3.6 Å resolution by cryo-electron microscopy and find small differences at the β-tubulin lateral interactions. Using in vitro reconstitution assays and quantitative biochemistry, we show that tubulin's free energy scales inversely with temperature. The observed weakening of lateral contacts and the low apparent activation energy for tubulin incorporation provide an explanation for the overall stability and higher growth rates of microtubules in cold-adapted frog species. This study thus broadens our conceptual framework for understanding microtubule dynamics and provides insights into how conserved cellular processes are tailored to different ecological niches.
リンク	Curr Biol / PubMed:39798564
手法	EM (単粒子)
解像度	3.0 - 3.6 Å
構造データ	50811 9fvj EMDB-50811, PDB-9fvj: Xenopus borealis undecorated microtubule - 15 protofilament, 3-start helix 手法: EM (単粒子) / 解像度: 3.2 Å 50941 9g0o EMDB-50941, PDB-9g0o: Xenopus borealis undecorated microtubule - 14 protofilament, 3-start helix 手法: EM (単粒子) / 解像度: 3.3 Å 50942 9g0p EMDB-50942, PDB-9g0p: Xenopus laevis undecorated microtubule - 14 protofilament, 3-start helix 手法: EM (単粒子) / 解像度: 3.0 Å 50943 9g0q EMDB-50943, PDB-9g0q: Xenopus laevis undecorated microtubule - 15 protofilament, 3-start helix 手法: EM (単粒子) / 解像度: 3.2 Å 50945 9g0r EMDB-50945, PDB-9g0r: Xenopus laevis undecorated microtubule - 15 protofilament, 4-start helix 手法: EM (単粒子) / 解像度: 3.1 Å 50946 9g0s EMDB-50946, PDB-9g0s: Xenopus tropicalis undecorated microtubule - 14 protofilament, 3-start helix 手法: EM (単粒子) / 解像度: 3.6 Å 50947 9g0t EMDB-50947, PDB-9g0t: Xenopus tropicalis undecorated microtubule - 15 protofilament, 3-start helix 手法: EM (単粒子) / 解像度: 3.5 Å
化合物	ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP / GDP, エネルギー貯蔵分子YM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	xenopus borealis (カエル) xenopus laevis (アフリカツメガエル) xenopus tropicalis (ネッタイツメガエル)
キーワード	PROTEIN FIBRIL / Microtubule Tubulin Cytoskeleton Filament