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TitleMetal-induced conformational changes in the Sabiá virus spike complex.
Journal, issue, pagesNat Microbiol, Vol. 10, Issue 9, Page 2221-2230, Year 2025
Publish dateAug 1, 2025
AuthorsHadas Cohen-Dvashi / Michael Katz / Ron Diskin /
PubMed AbstractHaemorrhagic fever viruses from the Arenaviridae are a source of concern owing to their potential to cause lethal outbreaks and the lack of effective therapeutics. While structures of spike proteins ...Haemorrhagic fever viruses from the Arenaviridae are a source of concern owing to their potential to cause lethal outbreaks and the lack of effective therapeutics. While structures of spike proteins from 'Old World' arenaviruses are available, the differences and similarities to 'New World' arenaviruses, such as the Sabiá virus, remain unclear owing to the lack of New World spike structures. Here we present the structure of the isolated spike complex from the Sabiá virus, which mediates viral attachment and entry to the host cells, using single-particle cryo-electron microscopy. We find two distinct conformational states of the spike, representing its native closed state at 2.6 Å resolution and an open state at 2.9 Å resolution that it assumes during cell entry. In addition, we show that the opening of the spike and subsequent cell entry are dependent on acidic pH and an unidentified metal ion. Our study suggests potential differences in the cell entry mechanisms of clade B arenaviruses compared with others in the Arenaviridae family.
External linksNat Microbiol / PubMed:40751015
MethodsEM (single particle)
Resolution2.44 - 2.92 Å
Structure data

50862

9fya

EMDB-50862, PDB-9fya:
Structure of the Sabia Virus spike complex in a closed conformation
Method: EM (single particle) / Resolution: 2.64 Å

50864

9fye

EMDB-50864: Structure of the Sabia Virus spike complex in a closed conformation
PDB-9fye: Structure of the Sabia Virus spike complex in an open conformation
Method: EM (single particle) / Resolution: 2.92 Å

50865

9fyg

EMDB-50865, PDB-9fyg:
Structure of the Sabia Virus spike complex H157M mutant in a closed conformation
Method: EM (single particle) / Resolution: 2.44 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-K:
Unknown entry

Source
  • sabia virus
KeywordsVIRAL PROTEIN / Class-I spike complex

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