EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	The ABC transporter MsbA in a dozen environments.
ジャーナル・号・ページ	Structure, Vol. 33, Issue 5, Page 916-923.e4, Year 2025
掲載日	2025年5月1日
著者	Lea Hoffmann / Anika Baier / Lara Jorde / Michael Kamel / Jan-Hannes Schäfer / Kilian Schnelle / Alischa Scholz / Dmitry Shvarev / Jaslyn E M M Wong / Kristian Parey / Dovile Januliene / Arne Moeller /
PubMed 要旨	High-resolution structure determination of membrane proteins typically requires reconstitution into artificial membrane mimics. The choice of the specific membrane substitute can strongly affect the ...High-resolution structure determination of membrane proteins typically requires reconstitution into artificial membrane mimics. The choice of the specific membrane substitute can strongly affect the protein's specific activity, stability, and conformational spectrum, potentially leading to errors or misinterpretation during analysis. The bacterial ATP-binding cassette transporter MsbA is a prominent example of such environment-specific bias. Here, we present a systematic analysis of the conformational spectrum of MsbA, stabilized in a dozen environments, using cryoelectron microscopy (cryo-EM), and show pronounced feedback between the membrane mimetics and the transporter. Detergents generally favor wide inward-facing conformations while nanodiscs induce narrower conformations. Notably, only in three tested environments, MsbA samples the full movement of the nucleotide-binding domains, including narrow and wide conformations. We expect this study to serve as a blueprint for other membrane proteins, even where a structural reaction to the hydrophobic environment is not directly visible but still critical for the proteins' function.
リンク	Structure / PubMed:40056915
手法	EM (単粒子)
解像度	3.3 - 4.4 Å
構造データ	50774 9fuq EMDB-50774, PDB-9fuq: MsbA in DDM inward-facing wide open 手法: EM (単粒子) / 解像度: 3.8 Å 50775 9fur EMDB-50775, PDB-9fur: MsbA in LMNG inward-facing narrow 手法: EM (単粒子) / 解像度: 3.8 Å 50776 9fus EMDB-50776, PDB-9fus: MsbA in LMNG inward-facing wide open 手法: EM (単粒子) / 解像度: 3.8 Å 50777 9fut EMDB-50777, PDB-9fut: MsbA in GDN inward-facing narrow 手法: EM (単粒子) / 解像度: 3.8 Å 50778 9fuu EMDB-50778, PDB-9fuu: MsbA in GDN inward-facing wide open 手法: EM (単粒子) / 解像度: 3.6 Å 50779 9fuv EMDB-50779, PDB-9fuv: MsbA in Triton inward-facing wide open 手法: EM (単粒子) / 解像度: 4.0 Å 50780 9fuw EMDB-50780, PDB-9fuw: MsbA in Triton inward-facing wide (+) open 手法: EM (単粒子) / 解像度: 3.8 Å 50782 9fuy EMDB-50782, PDB-9fuy: MsbA in UDM inward-facing wide open 手法: EM (単粒子) / 解像度: 4.1 Å 50783 9fuz EMDB-50783, PDB-9fuz: MsbA in UDM inward-facing wide (+) open 手法: EM (単粒子) / 解像度: 4.2 Å 50784 9fv0 EMDB-50784, PDB-9fv0: MsbA in MSP1D1 Nanodisc inward-facing narrow open 手法: EM (単粒子) / 解像度: 3.9 Å 50785 9fv1 EMDB-50785, PDB-9fv1: MsbA in MSP1D1 (EPL:PC) Nanodisc inward-facing narrow open 手法: EM (単粒子) / 解像度: 3.8 Å 50786 9fv2 EMDB-50786, PDB-9fv2: MsbA in MSP1E3D1 Nanodisc inward-facing narrow open 手法: EM (単粒子) / 解像度: 3.3 Å 50787 9fv3 EMDB-50787, PDB-9fv3: MsbA in MSP2N2 Nanodisc inward-facing narrow open 手法: EM (単粒子) / 解像度: 3.9 Å 50788 9fv4 EMDB-50788, PDB-9fv4: MsbA in peptidisc inward-facing narrow (-) open 手法: EM (単粒子) / 解像度: 3.8 Å 50789 9fv5 EMDB-50789, PDB-9fv5: MsbA in peptidisc inward-facing narrow open 手法: EM (単粒子) / 解像度: 3.9 Å 50790 9fv6 EMDB-50790, PDB-9fv6: MsbA in Amphipol A8-35 inward-facing wide open 手法: EM (単粒子) / 解像度: 3.8 Å 50791 9fv7 EMDB-50791, PDB-9fv7: MsbA in Amphipol A8-35 inward-facing wide (+) open 手法: EM (単粒子) / 解像度: 4.0 Å 50792 9fv8 EMDB-50792, PDB-9fv8: MsbA in Amphipol 18 inward-facing wide open 手法: EM (単粒子) / 解像度: 3.4 Å 50793 9fv9 EMDB-50793, PDB-9fv9: MsbA in Amphipol 18 inward-facing wide (+) open 手法: EM (単粒子) / 解像度: 3.6 Å 50794 9fva EMDB-50794, PDB-9fva: MsbA in MSP2N2 Nanodisc inward-facing wide open 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-3PE: 1,2-Distearoyl-sn-glycerophosphoethanolamine / DSPE / リン脂質YM ChemComp-JSG: (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-5-[(2~{S},3~{S},4~{R},5~{R},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-4-[(2~{R},3~{S},4~{R},5~{S},6~{R})-6-[(1~{S})-2-[(2~{S},3~{S},4~{S},5~{S},6~{R})-6-[(1~{S})-1,2-bis(oxidanyl)ethyl]-3,4,5-tris(oxidanyl)oxan-2-yl]oxy-1-oxidanyl-ethyl]-3,4-bis(oxidanyl)-5-phosphonooxy-oxan-2-yl]oxy-3-oxidanyl-5-phosphonooxy-oxan-2-yl]oxy-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid ChemComp-KDL*: (2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-carboxy-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(3~{R})-3-dodecanoyloxytetradecanoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(3~{R})-3-oxidanyltetradecanoyl]amino]-4-[(3~{R})-3-oxidanyltetradecanoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(3~{R})-3-tetradecanoyloxytetradecanoyl]oxy-oxan-2-yl]methoxy]-5-oxidanyl-oxan-4-yl]oxy-4,5-bis(oxidanyl)oxane-2-carboxylic acid
由来	escherichia coli (大腸菌)
キーワード	TRANSPORT PROTEIN / ABC transporter