EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of 3'-tRNA maturation by the human mitochondrial RNase Z complex.
ジャーナル・号・ページ	EMBO J, Vol. 43, Issue 24, Page 6573-6590, Year 2024
掲載日	2024年11月8日
著者	Genís Valentín Gesé / B Martin Hällberg /
PubMed 要旨	Maturation of human mitochondrial tRNA is essential for cellular energy production, yet the underlying mechanisms remain only partially understood. Here, we present several cryo-EM structures of the ...Maturation of human mitochondrial tRNA is essential for cellular energy production, yet the underlying mechanisms remain only partially understood. Here, we present several cryo-EM structures of the mitochondrial RNase Z complex (ELAC2/SDR5C1/TRMT10C) bound to different maturation states of mitochondrial tRNA, showing the molecular basis for tRNA-substrate selection and catalysis. Our structural insights provide a molecular rationale for the 5'-to-3' tRNA processing order in mitochondria, the 3'-CCA antideterminant effect, and the basis for sequence-independent recognition of mitochondrial tRNA substrates. Furthermore, our study links mutations in ELAC2 to clinically relevant mitochondrial diseases, offering a deeper understanding of the molecular defects contributing to these conditions.
リンク	EMBO J / PubMed:39516281 / PubMed Central
手法	EM (単粒子)
解像度	1.9 - 3.34 Å
構造データ	EMDB-19954: Human mitochondrial RNase Z with tRNA-His, ELAC2 focused map 手法: EM (単粒子) / 解像度: 3.07 Å EMDB-19955: Human mitochondrial RNase Z with tRNA-His, consensus map 手法: EM (単粒子) / 解像度: 2.78 Å EMDB-19956: Human mitochondrial RNase Z with tRNA-His precursor, consensus map 手法: EM (単粒子) / 解像度: 2.69 Å EMDB-19957: Human mitochondrial RNase Z with tRNA-His precursor, ELAC2 focused map 手法: EM (単粒子) / 解像度: 3.34 Å EMDB-19958: Human mitochondrial RNase Z with tRNA-His-CCA, consensus map 手法: EM (単粒子) / 解像度: 3.08 Å EMDB-19959: Human mitochondrial RNase Z with tRNA-His-CCA, ELAC2 focused map 手法: EM (単粒子) / 解像度: 3.2 Å 50050 9ey0 EMDB-50050, PDB-9ey0: Human mitochondrial RNase Z with tRNA-His 手法: EM (単粒子) / 解像度: 2.78 Å 50051 9ey1 EMDB-50051, PDB-9ey1: Human mitochondrial RNase Z with tRNA-His precursor 手法: EM (単粒子) / 解像度: 2.9 Å 50052 9ey2 EMDB-50052, PDB-9ey2: Human mitochondrial RNase Z with tRNA-His-CCA 手法: EM (単粒子) / 解像度: 2.96 Å 51230 9gch EMDB-51230, PDB-9gch: Human mitochondrial RNase Z with tRNA-His-CCA, SDR5C1/TRMT10C focus 手法: EM (単粒子) / 解像度: 1.9 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-SAM: S-ADENOSYLMETHIONINE ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE / S-アデノシルホモシステイン ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH*: WATER
由来	homo sapiens (ヒト)
キーワード	RNA BINDING PROTEIN / RNAseZ / ELAC2 / mitochondria / tRNA / RNase Z