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- EMDB-19957: Human mitochondrial RNase Z with tRNA-His precursor, ELAC2 focused map -

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Basic information

Entry
Database: EMDB / ID: EMD-19957
TitleHuman mitochondrial RNase Z with tRNA-His precursor, ELAC2 focused map
Map dataHuman mitochondrial RNAseZ with tRNA-His precursor, ELAC2 focused map
Sample
  • Complex: RNAseZ with mt-tRNA-His
KeywordsRNAseZ / ELAC2 / mitochondria / tRNA / RNA BINDING PROTEIN
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.34 Å
AuthorsValentin Gese G / Hallberg BM
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: Embo J. / Year: 2024
Title: Structural basis of 3'-tRNA maturation by the human mitochondrial RNase Z complex
Authors: Valentin Gese G / Hallberg BM
History
DepositionMar 26, 2024-
Header (metadata) releaseNov 13, 2024-
Map releaseNov 13, 2024-
UpdateNov 13, 2024-
Current statusNov 13, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_19957.png

Downloads & links

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Map

FileDownload / File: emd_19957.map.gz / Format: CCP4 / Size: 274.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationHuman mitochondrial RNAseZ with tRNA-His precursor, ELAC2 focused map
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.01 Å/pix.
x 416 pix.
= 420.16 Å		1.01 Å/pix.
x 416 pix.
= 420.16 Å		1.01 Å/pix.
x 416 pix.
= 420.16 Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.01 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.3
Minimum - Maximum-1.1503394 - 1.8553671
Average (Standard dev.)-0.00030056346 (±0.041028116)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions416416416
Spacing416416416
CellA=B=C: 420.16 Å
α=β=γ: 90.0 °

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Supplemental data

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Half map: Human mitochondrial RNAseZ with tRNA-His precursor, ELAC2 focused...

Fileemd_19957_half_map_1.map
AnnotationHuman mitochondrial RNAseZ with tRNA-His precursor, ELAC2 focused half map 2
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Human mitochondrial RNAseZ with tRNA-His precursor, ELAC2 focused...

Fileemd_19957_half_map_2.map
AnnotationHuman mitochondrial RNAseZ with tRNA-His precursor, ELAC2 focused half map 1
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : RNAseZ with mt-tRNA-His

EntireName: RNAseZ with mt-tRNA-His
Components
  • Complex: RNAseZ with mt-tRNA-His

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Supramolecule #1: RNAseZ with mt-tRNA-His

SupramoleculeName: RNAseZ with mt-tRNA-His / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 250 KDa

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.5
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average electron dose: 48.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: SPOT SCAN / Imaging mode: BRIGHT FIELD / Nominal defocus max: 2.0 µm / Nominal defocus min: 0.4 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 643629
Startup modelType of model: INSILICO MODEL
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.34 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 15961
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.3.1)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: cryoSPARC (ver. 4.3.1)
FSC plot (resolution estimation)

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Atomic model buiding 1

Initial model
PDB IDChain

7onu

source_name: PDB, initial_model_type: experimental model

bq52

source_name: AlphaFold, initial_model_type: in silico model
RefinementSpace: REAL / Protocol: FLEXIBLE FIT

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