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TitleCryo-EM reveals an ensemble of cytochrome P450 reductase conformations in solution.
Journal, issue, pagesProtein Sci, Vol. 35, Issue 2, Page e70448, Year 2026
Publish dateJan 21, 2026
AuthorsGalina I Lepesheva / Tatiana Y Hargrove / Yi Ren /
PubMed AbstractThe eukaryotic electron transport system, mediated by cytochrome P450 reductase (CPR), plays a crucial role in driving myriads of reactions involved in the biosynthesis of physiologically active ...The eukaryotic electron transport system, mediated by cytochrome P450 reductase (CPR), plays a crucial role in driving myriads of reactions involved in the biosynthesis of physiologically active compounds (such as sterols, steroids, vitamins, and natural products), as well as in the metabolism of drugs, toxins, and carcinogens. CPR is a diflavin-containing enzyme found ubiquitously on the cytosolic side of the endoplasmic reticulum. While several crystal structures of CPR are available, its conformational states in solution, along with the molecular details of action, remain debatable. Here, we determined the 3.3 Å cryo-EM structure of rat CPR, marking the first electron microscopy structure of this relatively small protein (77 kDa). In this structure, the full-length, fully active enzyme adopts a compact conformation, which, however, is more relaxed than in crystal structures. Moreover, we structurally characterized less populated variations of compact CPR conformations and identified a fraction of molecules (~20%) with the FMN-binding domain either not visible or positioned far from the rest of the catalytic core. These results support the idea that large-scale interdomain rearrangements serve as the structural basis for CPR function and suggest that cryo-EM techniques can help uncover the intricate molecular mechanisms governing the CPR-mediated electron transfer cycle.
External linksProtein Sci / PubMed:41562286 / PubMed Central
MethodsEM (single particle)
Resolution3.33 Å
Structure data

40857

9ef0

EMDB-40857: Cryo-EM map of cytochrome P450 reductase
PDB-9ef0: EM structure of cytochrome P450 reductase
Method: EM (single particle) / Resolution: 3.33 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

Source
  • rattus norvegicus (Norway rat)
KeywordsOXIDOREDUCTASE / cytochrome P450 reductase / CPR / POR / electron transfer / endoplasmic reticulum / flavoprotein

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