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TitleConformational regulation of two essential activators of bacterial cell elongation.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 122, Issue 45, Page e2514198122, Year 2025
Publish dateNov 11, 2025
AuthorsMorgan S A Gilman / Irina Shlosman / Daniel D Samé Guerra / Masy Domecillo / Elayne M Fivenson / Claire Bourett / Thomas G Bernhardt / Nicholas F Polizzi / Joseph J Loparo / Andrew C Kruse /
PubMed AbstractThe peptidoglycan (PG) cell wall is critical for bacterial growth and survival and is a primary antibiotic target. MreD is an essential accessory factor of the Rod complex, which carries out PG ...The peptidoglycan (PG) cell wall is critical for bacterial growth and survival and is a primary antibiotic target. MreD is an essential accessory factor of the Rod complex, which carries out PG synthesis during elongation, yet little is known about how MreD facilitates this process. Here, we present the cryoelectron microscopy structure of MreD in complex with another essential Rod complex component, MreC. The structure reveals that a periplasmic-facing pocket of MreD interacts with multiple membrane-proximal regions of MreC. We use single-molecule FRET to show that MreD controls the conformation of MreC through these contacts, inducing a state primed for Rod complex activation. Using as a model, we demonstrate that disrupting these interactions abolishes Rod complex activity in vivo. Our findings reveal the role of MreD in bacterial cell shape determination and highlight its potential as an antibiotic target.
External linksProc Natl Acad Sci U S A / PubMed:41183199 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 7.4 Å
Structure data

47199

9dvb

EMDB-47199: Thermus thermophilus MreC-MreD complex with an internal MreD BRIL fusion and an anti-BRIL Fab.
PDB-9dvb: Thermus thermophilus MreC-MreD complex with an internal MreD BRIL fusion and an anti-BRIL Fab
Method: EM (single particle) / Resolution: 3.6 Å

47200

9dvc

EMDB-47200: Thermus thermophilus MreC-MreD complex with a C-terminal MreD BRIL fusion and an anti-BRIL Fab.
PDB-9dvc: Thermus thermophilus MreC-MreD complex with a C-terminal MreD BRIL fusion and an anti-BRIL Fab
Method: EM (single particle) / Resolution: 7.4 Å

Source
  • thermus thermophilus (bacteria)
  • escherichia coli (E. coli)
  • synthetic construct (others)
KeywordsMEMBRANE PROTEIN / Peptidoglycan synthesis regulator / S-component fold / Rod complex / SEDS activator

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