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TitleDesign and evolution of the tetracycline repressor into sulfonylurea herbicide-responsive gene switches for field crops.
Journal, issue, pagesNat Commun, Year 2026
Publish dateSep 30, 2024 (structure data deposition date)
AuthorsMcBride, K.E. / Kakani, N.K. / Bozhanova, N.G. / Fang, J. / Lowe, K. / Leija, C. / McGonigle, B. / Schreiter, E.R. / Hermanson, F. / Chan, M. ...McBride, K.E. / Kakani, N.K. / Bozhanova, N.G. / Fang, J. / Lowe, K. / Leija, C. / McGonigle, B. / Schreiter, E.R. / Hermanson, F. / Chan, M. / Arling, M. / Wang, N. / Cho, H.J. / Richey, C. / Hoerster, G. / Marvin, J.S. / Lenderts, B. / McReynolds, M.R. / Hastings, C. / Madrigal, A. / Falco, S.C. / Lassner, M.W. / Anand, A. / Gordon-Kamm, W. / Looger, L.L.
External linksNat Commun / PubMed:42259793
MethodsX-ray diffraction
Resolution2 - 3.1 Å
Structure data

PDB-9dt2:
Crystal structure of the engineered sulfonylurea repressor EsR (L7-D1), apo form
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-9dt3:
Crystal structure of the engineered sulfonylurea repressor EsR (L11-C6), bound to ethametsulfuron-methyl
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-9dt4:
Crystal structure of the engineered sulfonylurea repressor CsR (L4.2-20), apo form
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-9dt5:
Crystal structure of the engineered sulfonylurea repressor CsR (L4.2-20), bound to chlorsulfuron
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-RXF:
methyl 2-[[4-ethoxy-6-(methylamino)-1,3,5-triazin-2-yl]carbamoylsulfamoyl]benzoate

ChemComp-HOH:
WATER

ChemComp-1CS:
1-(2-CHLOROPHENYLSULFONYL)-3-(4-METHOXY-6-METHYL-L,3,5-TRIAZIN-2-YL)UREA

Source
  • escherichia coli (E. coli)
KeywordsDNA BINDING PROTEIN / engineered / ligand / repressor / transcription / TetR

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