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TitleDesign of light- and chemically responsive protein assemblies through host-guest interactions.
Journal, issue, pagesChem, Vol. 11, Issue 6, Year 2025
Publish dateJun 12, 2025
AuthorsZhiyin Zhang / Huat T Chiang / Ying Xia / Nicole Avakyan / Ravi R Sonani / Fengbin Wang / Edward H Egelman / James J De Yoreo / Lilo D Pozzo / F Akif Tezcan /
PubMed AbstractHost-guest interactions have been widely used to build responsive materials and molecular machines owing to their inherently dynamic nature, interaction specificity, and responsiveness to diverse ...Host-guest interactions have been widely used to build responsive materials and molecular machines owing to their inherently dynamic nature, interaction specificity, and responsiveness to diverse stimuli. Here we have set out to exploit these advantages of host-guest chemistry in the design of dynamic protein assemblies, using a symmetric protein, RhuA, as a building block. We show that RhuA variant individually modified with β-cyclodextrin (βCD) (host) or azobenzene (guest) functionalities can specifically pair with each other to form highly ordered 1- and 2-D assemblies. Association and dissociation RhuA-RhuA assemblies can be controlled by UV and visible light as well as by small-molecule modulators of βCD-azobenzene interactions. Kinetics analyses reveal that RhuA-RhuA nanotubes assemble without a nucleation barrier, a highly unusual occurrence for helical supramolecular systems. Taken together, our findings provide a compelling example for achieving complex structural and dynamic outcomes in protein assembly through simple chemical design.
External linksChem / PubMed:40862094 / PubMed Central
MethodsEM (helical sym.) / EM (single particle)
Resolution3.03 - 7.8 Å
Structure data

EMDB-46767: azoRhuA-bCDRhuA co-assembled nanotubes, 10-start
Method: EM (helical sym.) / Resolution: 7.8 Å

EMDB-46768: azoRhuA-bCDRhuA co-assembled nanotubes, 11-start
Method: EM (helical sym.) / Resolution: 6.8 Å

EMDB-46769: azoRhuA-bCDRhuA co-assembled nanotubes, 12-start
Method: EM (helical sym.) / Resolution: 7.7 Å

46826

9dgh

EMDB-46826, PDB-9dgh:
Focused region on azoRhuA-bCDRhuA co-assembled nanotubes
Method: EM (single particle) / Resolution: 3.03 Å

Chemicals

ChemComp-ZN:
Unknown entry

PDB-1a4g:
INFLUENZA VIRUS B/BEIJING/1/87 NEURAMINIDASE COMPLEXED WITH ZANAMIVIR

Source
  • escherichia coli (E. coli)
KeywordsLYASE / Assembly

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