EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis of nucleosome recognition by the conserved Dsup and HMGN nucleosome-binding motif.
ジャーナル・号・ページ	Genes Dev, Vol. 39, Issue 19-20, Page 1155-1161, Year 2025
掲載日	2025年10月1日
著者	Jaime Alegrio-Louro / Grisel Cruz-Becerra / George A Kassavetis / James T Kadonaga / Andres E Leschziner /
PubMed 要旨	The tardigrade damage suppressor (Dsup) and vertebrate high-mobility group N (HMGN) proteins bind specifically to nucleosomes via a conserved motif whose structure has not been experimentally ...The tardigrade damage suppressor (Dsup) and vertebrate high-mobility group N (HMGN) proteins bind specifically to nucleosomes via a conserved motif whose structure has not been experimentally determined. Here we used cryo-EM to show that both proteins bind to the nucleosome acidic patch via analogous arginine anchors with one molecule bound to each face of the nucleosome. We additionally used the natural promoter-containing 5S rDNA sequence for structural analysis of the nucleosome. These structures of an ancient nucleosome-binding motif suggest that there is an untapped realm of proteins with a related mode of binding to chromatin.
リンク	Genes Dev / PubMed:40721296 / PubMed Central
手法	EM (単粒子)
解像度	2.5 - 3.3 Å
構造データ	46536 9d3k EMDB-46536, PDB-9d3k: Two Dsup molecules in complex with the nucleosome open from both sides 手法: EM (単粒子) / 解像度: 2.7 Å 46537 9d3l EMDB-46537, PDB-9d3l: Two Dsup molecules in complex with the nucleosome open from the left side 手法: EM (単粒子) / 解像度: 2.8 Å 46538 9d3m EMDB-46538, PDB-9d3m: Two HMGN2s in complex with the nucleosome 手法: EM (単粒子) / 解像度: 2.9 Å 46540 9d3n EMDB-46540, PDB-9d3n: 167-bp 5S rDNA nucleosome cross-linked with glutaraldehyde 手法: EM (単粒子) / 解像度: 3.0 Å 46542 9d3o EMDB-46542, PDB-9d3o: 167-bp 5S rDNA nucleosome - closed 手法: EM (単粒子) / 解像度: 3.0 Å 46543 9d3p EMDB-46543, PDB-9d3p: 167-bp 5S rDNA nucleosome - open I (open only on the downstream side) 手法: EM (単粒子) / 解像度: 2.5 Å 46544 9d3q EMDB-46544, PDB-9d3q: 167-bp 5S rDNA nucleosome - open II 手法: EM (単粒子) / 解像度: 2.8 Å 46545 9d3r EMDB-46545, PDB-9d3r: 147-bp 5S rDNA nucleosome - closed 手法: EM (単粒子) / 解像度: 3.3 Å 46546 9d3s EMDB-46546, PDB-9d3s: 147-bp 5S rDNA nucleosome - open I (open on the downstream side) 手法: EM (単粒子) / 解像度: 3.1 Å 46547 9d3t EMDB-46547, PDB-9d3t: 147-bp 5S rDNA nucleosome cross-linked with glutaraldehyde 手法: EM (単粒子) / 解像度: 2.8 Å
由来	xenopus borealis (カエル) synthetic construct (人工物) homo sapiens (ヒト) ramazzottius varieornatus (無脊椎動物)
キーワード	GENE REGULATION/DNA / Dsup / nucleosome / tardigrade damage supressor protein / GENE REGULATION / GENE REGULATION-DNA complex / high mobility group N proteins (HMGN proteins) / HMGN2 / non-histone chromosomal proteins / 5S rDNA nucleosome / natural nucleosome positioning sequence / glutaraldehyde / cross-linking / DNA sequence-dependent breathing