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TitleCryo-EM analysis of Pseudomonas phage Pa193 structural components.
Journal, issue, pagesCommun Biol, Vol. 7, Issue 1, Page 1275, Year 2024
Publish dateOct 6, 2024
AuthorsStephano M Iglesias / Chun-Feng David Hou / Johnny Reid / Evan Schauer / Renae Geier / Angela Soriaga / Lucy Sim / Lucy Gao / Julian Whitelegge / Pierre Kyme / Deborah Birx / Sebastien Lemire / Gino Cingolani /
PubMed AbstractThe World Health Organization has designated Pseudomonas aeruginosa as a critical pathogen for the development of new antimicrobials. Bacterial viruses, or bacteriophages, have been used in various ...The World Health Organization has designated Pseudomonas aeruginosa as a critical pathogen for the development of new antimicrobials. Bacterial viruses, or bacteriophages, have been used in various clinical settings, commonly called phage therapy, to address this growing public health crisis. Here, we describe a high-resolution structural atlas of a therapeutic, contractile-tailed Pseudomonas phage, Pa193. We used bioinformatics, proteomics, and cryogenic electron microscopy single particle analysis to identify, annotate, and build atomic models for 21 distinct structural polypeptide chains forming the icosahedral capsid, neck, contractile tail, and baseplate. We identified a putative scaffolding protein stabilizing the interior of the capsid 5-fold vertex. We also visualized a large portion of Pa193 ~ 500 Å long tail fibers and resolved the interface between the baseplate and tail fibers. The work presented here provides a framework to support a better understanding of phages as biomedicines for phage therapy and inform engineering opportunities.
External linksCommun Biol / PubMed:39370451 / PubMed Central
MethodsEM (single particle)
Resolution2.9 - 3.5 Å
Structure data

44163

9b40

EMDB-44163, PDB-9b40:
Pseudomonas phage Pa193 5-fold vertex (capsid, decorating, and scaffolding proteins)
Method: EM (single particle) / Resolution: 2.9 Å

44164

9b41

EMDB-44164, PDB-9b41:
Pseudomonas phage Pa193 Neck (portal and head-to-tail proteins)
Method: EM (single particle) / Resolution: 3.2 Å

44166

9b42

EMDB-44166, PDB-9b42:
Pseudomonas phage Pa193 neck and extended tail (collar, gateway, tail tube, and sheath proteins)
Method: EM (single particle) / Resolution: 3.5 Å

44168

9b45

EMDB-44168, PDB-9b45:
Pseudomonas phage Pa193 baseplate complex and tail fiber
Method: EM (single particle) / Resolution: 3.3 Å

Source
  • pseudomonas virus pa193
KeywordsVIRAL PROTEIN / phage / bacteriophage / gene product 24 (gp24) / STRUCTURAL PROTEIN / gene product 25 (gp25) / scaffolding protein / decorating protein / major capsid protein / gene product 26 (gp26) / gene product (gp19) / gene product 28 (gp28) / head-to-tail protein / portal protein / gene product (gp29) / gene product 30 (gp30) / collar protein / gateway protein / sheath protein / tail tube protein / gene product 32 (gp32) / gene product 33 (gp33) / baseplate complex / tail fiber

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