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Structure paper

TitleStructural and Functional Basis of GenB2 Isomerase Activity from Gentamicin Biosynthesis.
Journal, issue, pagesAcs Chem. Biol., Year 2024
Publish dateFeb 28, 2024 (structure data deposition date)
AuthorsOliveira, G.S. / Dos S Bury, P. / Huang, F. / Li, Y. / Araujo, N.C. / Zhou, J. / Sun, Y. / Leeper, F.J. / Leadlay, P.F. / Dias, M.V.B.
External linksAcs Chem. Biol. / PubMed:39207862
MethodsX-ray diffraction
Resolution1.35 - 1.66 Å
Structure data

PDB-9au3:
Crystal structure of GenB2 in complex with G418
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-9aue:
Crystal structure of the holo form of GenB2 in complex with PMP
Method: X-RAY DIFFRACTION / Resolution: 1.46 Å

PDB-9b0c:
Crystal structure of GenB2 in complex with gentamicin X2.
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

Chemicals

ChemComp-PMP:
4'-DEOXY-4'-AMINOPYRIDOXAL-5'-PHOSPHATE

ChemComp-GET:
GENETICIN / antibiotic*YM

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-GOL:
GLYCEROL

ChemComp-827:
(1R,2S,3S,4R,6S)-4,6-diamino-3-{[3-deoxy-4-C-methyl-3-(methylamino)-beta-L-arabinopyranosyl]oxy}-2-hydroxycyclohexyl 2-amino-2-deoxy-alpha-D-glucopyranoside

Source
  • micromonospora echinospora (bacteria)
KeywordsANTIBIOTIC / Gentamicin biosynthesis / G418 / Pyridoxamine-5'-phosphate (PMP) / holo form / BIOSYNTHETIC PROTEIN / gentamicin / PLP-dependent enzymes

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