EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for malate-driven, pore lipid-regulated activation of the Arabidopsis vacuolar anion channel ALMT9.
ジャーナル・号・ページ	Nat Commun, Vol. 16, Issue 1, Page 1817, Year 2025
掲載日	2025年2月20日
著者	Yeongmok Lee / Elsa Demes-Causse / Jaemin Yoo / Seo Young Jang / Seoyeon Jung / Justyna Jaślan / Geum-Sook Hwang / Jejoong Yoo / Alexis De Angeli / Sangho Lee /
PubMed 要旨	In plant cells, ALMTs are key plasma and vacuolar membrane-localized anion channels regulating plant responses to the environment. Vacuolar ALMTs control anion accumulation in plant cells and, in ...In plant cells, ALMTs are key plasma and vacuolar membrane-localized anion channels regulating plant responses to the environment. Vacuolar ALMTs control anion accumulation in plant cells and, in guard cells, they regulate stomata aperture. The activation of vacuolar ALMTs depends on voltage and cytosolic malate, but the underlying molecular mechanisms remain elusive. Here we report the cryo-EM structures of ALMT9 from Arabidopsis thaliana (AtALMT9), a malate-activated vacuolar anion channel, in plugged and unplugged lipid-bound states. In all these states, membrane lipids interact with the ion conduction pathway of AtALMT9. We identify two unplugged states presenting two distinct pore width profiles. Combining structural and functional analysis we identified conserved residues involved in ion conduction and in the pore lipid interaction. Molecular dynamics simulations revealed a peculiar anion conduction mechanism in AtALMT9. We propose a voltage-dependent activation mechanism based on the competition between pore lipids and malate at the cytosolic entrance of the channel.
リンク	Nat Commun / PubMed:39979303 / PubMed Central
手法	EM (単粒子)
解像度	2.63 - 3.7 Å
構造データ	60459 8zte EMDB-60459, PDB-8zte: AtALMT9 with LMNG and sterol mimic CHS (sterol1 class) 手法: EM (単粒子) / 解像度: 3.17 Å 60461 8ztg EMDB-60461, PDB-8ztg: AtALMT9 with LMNG and sterol mimic CHS (sterol2 class) 手法: EM (単粒子) / 解像度: 2.95 Å 60462 8zth EMDB-60462, PDB-8zth: AtALMT9 with LMNG (narrow class) 手法: EM (単粒子) / 解像度: 2.63 Å 60463 8zti EMDB-60463, PDB-8zti: AtALMT9 with LMNG (wide class) 手法: EM (単粒子) / 解像度: 2.9 Å 60464 8ztj EMDB-60464, PDB-8ztj: AtALMT9 with LMNG (cis1-PI4P class) 手法: EM (単粒子) / 解像度: 2.73 Å 60465 8ztk EMDB-60465, PDB-8ztk: AtALMT9 with LMNG (cis2 class) 手法: EM (単粒子) / 解像度: 2.78 Å 60466 8ztl EMDB-60466, PDB-8ztl: AtALMT9 with LMNG (intermediate class) 手法: EM (単粒子) / 解像度: 2.75 Å 60467 8ztm EMDB-60467, PDB-8ztm: AtALMT9 with LMNG (trans1 class) 手法: EM (単粒子) / 解像度: 2.84 Å 60468 8ztn EMDB-60468, PDB-8ztn: AtALMT9 with LMNG (trans2 class) 手法: EM (単粒子) / 解像度: 2.77 Å 61818 9jtw EMDB-61818, PDB-9jtw: AtALMT1 with LMNG and sterol mimic CHS 手法: EM (単粒子) / 解像度: 3.7 Å
化合物	ChemComp-Y01: CHOLESTEROL HEMISUCCINATE ChemComp-3PH: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE ChemComp-MLT: D-MALATE ChemComp-HOH: WATER ChemComp-D10: DECANE ChemComp-PLM: PALMITIC ACID ChemComp-OCT: N-OCTANE ChemComp-STE: STEARIC ACID PDB-1l2f: Crystal structure of NusA from Thermotoga maritima: a structure-based role of the N-terminal domain ChemComp-LPP: 2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / リン脂質*YM
由来	arabidopsis thaliana (シロイヌナズナ)
キーワード	MEMBRANE PROTEIN / Plant / Stomata / Vacuole / ALMT / Ion channel / Channel