Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Cryo-EM and cryo-ET reveal the molecular architecture and host interactions of mycobacteriophage Douge.
Journal, issue, pages	Cell Rep, Vol. 44, Issue 8, Page 116057, Year 2025
Publish date	Aug 26, 2025
Authors	Jitendra Maharana / Chun-Hsiung Wang / Li-An Tsai / Yi-Ting Liao / Cheng-Han Yang / Melvin C Shen / Lourriel S Macale / Thang Ngoc Tran / Joemark Narsico / Ronelito J Perez / Sunil Kumar Tewary / Jian-Li Wu / Hong-You Lin / Shu-Wei Chang / Aaron Franklin / Patrick J Moynihan / Deborah Jacobs-Sera / Krista G Freeman / Graham F Hatfull / Todd L Lowary / Meng-Chiao Ho /
PubMed Abstract	Recent reports highlight the efficacy of engineered mycobacteriophages to treat non-tuberculosis mycobacterial disease. Molecular insights into mycobacteriophage architecture and host interactions ...Recent reports highlight the efficacy of engineered mycobacteriophages to treat non-tuberculosis mycobacterial disease. Molecular insights into mycobacteriophage architecture and host interactions could allow structure-guided phage engineering to increase efficacy and broaden host range, but such information is currently unavailable. We describe the cryoelectron microscopy (cryo-EM) structure of mycobacteriophage Douge, which contains 1,105 protein subunits assembled into a complete siphophage and is coated with glycan-binding domains for mycobacterial cell surface interactions. When filled with viral genome, the channel spanning the connector, tail, and baseplate is sealed by tape measure proteins, providing a genome gating system and requiring limited structural changes for genome ejection upon phage-host contact. Nanometer-resolution cryoelectron tomography (cryo-ET) snapshots of phage-host interactions show that the baseplate remains attached to the mycobacterial outer membrane during viral genome ejection. This study reveals high-resolution structural details of this mycobacteriophage and its interaction with host glycans.
External links	Cell Rep / PubMed:40711877
Methods	EM (single particle)
Resolution	2.18 - 6.03 Å
Structure data	39973 8zdh EMDB-39973, PDB-8zdh: Cryo-EM structure of Mycobacteriophage Douge genome-packed capsid (gp8 and gp113) Method: EM (single particle) / Resolution: 3.2 Å EMDB-39974: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed capsid (Penton region: gp8) Method: EM (single particle) / Resolution: 2.95 Å EMDB-39975: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed capsid (P-hexon region: gp8 and gp113) Method: EM (single particle) / Resolution: 2.83 Å EMDB-39976: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed capsid (C-hexon region: gp8 and gp113) Method: EM (single particle) / Resolution: 2.8 Å EMDB-39977: Composite cryo-EM map of Mycobacteriophage Douge genome-packed capsid (Penton, P-hexon and C-hexon region: gp8 and gp113) Method: EM (single particle) / Resolution: 2.86 Å 39978 8zdi EMDB-39978, PDB-8zdi: Cryo-EM structure of Mycobacteriophage Douge genome-free capsid (gp8) Method: EM (single particle) / Resolution: 3.58 Å EMDB-39979: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free capsid (Penton region: gp8) Method: EM (single particle) / Resolution: 3.37 Å EMDB-39980: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free capsid (P-hexon region: gp8) Method: EM (single particle) / Resolution: 3.16 Å EMDB-39981: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free capsid (C-hexon region: gp8) Method: EM (single particle) / Resolution: 3.12 Å EMDB-39982: Composite cryo-EM map of Mycobacteriophage Douge genome-free capsid (Penton, P-hexon and C-hexon region: gp8) Method: EM (single particle) / Resolution: 3.22 Å 39983 8zdj EMDB-39983, PDB-8zdj: Cryo-EM structure of Mycobacteriophage Douge genome-packed connector (gp5, gp9, gp10, gp12 and gp13) Method: EM (single particle) / Resolution: 3.74 Å 39984 8zdk EMDB-39984, PDB-8zdk: Cryo-EM structure of Mycobacteriophage Douge genome-packed vertex (gp8 and gp113) Method: EM (single particle) / Resolution: 3.44 Å EMDB-39985: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed connector (gp5 and gp9) Method: EM (single particle) / Resolution: 2.82 Å EMDB-39986: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed connector (gp10, gp12 and gp13) Method: EM (single particle) / Resolution: 2.96 Å EMDB-39987: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-packed connector (gp8 and gp113) Method: EM (single particle) / Resolution: 3.24 Å EMDB-39988: Composite cryo-EM map of Mycobacteriophage Douge genome-packed connector-vertex (gp5, gp9, gp10, gp12, gp13, gp8 and gp113) Method: EM (single particle) / Resolution: 3.24 Å 39989 8zdl EMDB-39989, PDB-8zdl: Cryo-EM structure of Mycobacteriophage Douge genome-free connector (gp5, gp9, gp10, gp12 and gp13) Method: EM (single particle) / Resolution: 3.78 Å 39990 8zdm EMDB-39990, PDB-8zdm: Cryo-EM structure of Mycobacteriophage Douge genome-free vertex (gp8) Method: EM (single particle) / Resolution: 4.22 Å EMDB-39991: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free connector (gp5 and gp9) Method: EM (single particle) / Resolution: 3.53 Å EMDB-39992: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free connector (gp10, gp12 and gp13) Method: EM (single particle) / Resolution: 3.89 Å EMDB-39993: Focused refinement cryo-EM map of Mycobacteriophage Douge genome-free connector (gp8) Method: EM (single particle) / Resolution: 4.0 Å EMDB-39994: Composite cryo-EM map of Mycobacteriophage Douge genome-free connector-vertex (gp5, gp9, gp10, gp12, gp13 and gp8) Method: EM (single particle) / Resolution: 4.0 Å 39995 8zdn EMDB-39995, PDB-8zdn: Cryo-EM structure of Mycobacteriophage Douge genome-free tail tube (gp13) Method: EM (single particle) / Resolution: 3.07 Å 39996 8zdo EMDB-39996, PDB-8zdo: Cryo-EM structure of Mycobacteriophage Douge baseplate (gp13, gp17, gp23, gp16, gp18 and gp20) Method: EM (single particle) / Resolution: 2.97 Å EMDB-39997: Focused refinement cryo-EM map of Mycobacteriophage Douge baseplate (gp13) Method: EM (single particle) / Resolution: 2.71 Å EMDB-39998: Focused refinement cryo-EM map of Mycobacteriophage Douge baseplate (gp17 and gp23) Method: EM (single particle) / Resolution: 2.75 Å EMDB-39999: Focused refinement cryo-EM map of Mycobacteriophage Douge baseplate (gp16, gp18 and gp20) Method: EM (single particle) / Resolution: 2.63 Å EMDB-60000: Composite cryo-EM map of Mycobacteriophage Douge baseplate (gp13, gp17, gp23, gp16, gp18 and gp20) Method: EM (single particle) / Resolution: 2.7 Å 60001 8zdp EMDB-60001, PDB-8zdp: Cryo-EM structure of Mycobacteriophage Douge Central fiber (gp20) Method: EM (single particle) / Resolution: 4.26 Å EMDB-60002: Focused refinement cryo-EM map of Mycobacteriophage Douge Central fiber (gp20, top region) Method: EM (single particle) / Resolution: 4.48 Å EMDB-60003: Focused refinement cryo-EM map of Mycobacteriophage Douge central fiber (gp20, bottom region) Method: EM (single particle) / Resolution: 3.88 Å EMDB-60004: Composite cryo-EM map of Mycobacteriophage Douge central fiber (gp20) Method: EM (single particle) / Resolution: 4.18 Å 60005 8zdq EMDB-60005, PDB-8zdq: Cryo-EM structure of Mycobacteriophage Douge complete baseplate (gp13, gp17, gp23, gp16, gp18 and gp20) Method: EM (single particle) / Resolution: 3.29 Å 60024 8zea EMDB-60024, PDB-8zea: Cryo-EM structure of Mycobacteriophage Douge genome-packed tail tube (gp13) Method: EM (single particle) / Resolution: 2.18 Å EMDB-60714: Cryo-EM structure of Mycobacteriophage Douge genome-packed connector-vertex (gp5, gp8, gp9, gp10, gp12, gp13 and gp113 Method: EM (single particle) / Resolution: 3.74 Å EMDB-60715: Cryo-EM structure of Mycobacteriophage Douge genome-free connector vertex (gp5, gp8, gp9, gp10, gp12 and gp13) Method: EM (single particle) / Resolution: 6.03 Å
Source	mycolicibacterium smegmatis mc2 155 (bacteria)
Keywords	VIRUS / Mycobacteriophage / phage structure / Icosahedral / cryo-EM / protein assembly / VIRAL PROTEIN