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TitleThe binding mechanism of an anti-multiple myeloma antibody to the human GPRC5D homodimer.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 5255, Year 2024
Publish dateJun 19, 2024
AuthorsPengfei Yan / Xi Lin / Lijie Wu / Lu Xu / Fei Li / Junlin Liu / Fei Xu /
PubMed AbstractGPRC5D is an atypical Class C orphan G protein-coupled receptor. Its high expression on the surface of multiple myeloma cells has rendered it an attractive target for therapeutic interventions, ...GPRC5D is an atypical Class C orphan G protein-coupled receptor. Its high expression on the surface of multiple myeloma cells has rendered it an attractive target for therapeutic interventions, including monoclonal antibodies, CAR-T cells, and T-cell engagers. Despite its therapeutic potential, the insufficient understanding regarding of the receptor's structure and antibody recognition mechanism has impeded the progress of effective therapeutic development. Here, we present the structure of GPRC5D in complex with a preclinical-stage single-chain antibody (scFv). Our structural analysis reveals that the GPRC5D presents a close resemblance to the typical Class C GPCRs in the transmembrane region. We identify a distinct head-to-head homodimer arrangement and interface mainly involving TM4, setting it apart from other Class C homo- or hetero-dimers. Furthermore, we elucidate the binding site engaging a sizable extracellular domain on GPRC5D for scFv recognition. These insights not only unveil the distinctive dimer organization of this unconventional Class C GPCR but also hold the potential to advance drug development targeting GPRC5D for the treatment of multiple myeloma.
External linksNat Commun / PubMed:38898050 / PubMed Central
MethodsEM (single particle)
Resolution3.4 Å
Structure data

39696

8yzk

EMDB-39696, PDB-8yzk:
Orphan receptor GPRC5D in complex with scFv150-18
Method: EM (single particle) / Resolution: 3.4 Å

Source
  • homo sapiens (human)
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / orphan receptor / GPRC5D complex / scFv

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