+Search query
-Structure paper
Title | The binding and structural basis of fox ACE2 to RBDs from different sarbecoviruses. |
---|---|
Journal, issue, pages | Virol Sin, Vol. 39, Issue 4, Page 609-618, Year 2024 |
Publish date | Jun 10, 2024 |
![]() | Junsen Chen / Junqing Sun / Zepeng Xu / Linjie Li / Xinrui Kang / Chunliang Luo / Qi Wang / Xueyang Guo / Yan Li / Kefang Liu / Ying Wu / ![]() |
PubMed Abstract | Foxes are susceptible to SARS-CoV-2 in laboratory settings, and there have also been reports of natural infections of both SARS-CoV and SARS-CoV-2 in foxes. In this study, we assessed the binding ...Foxes are susceptible to SARS-CoV-2 in laboratory settings, and there have also been reports of natural infections of both SARS-CoV and SARS-CoV-2 in foxes. In this study, we assessed the binding capacities of fox ACE2 to important sarbecoviruses, including SARS-CoV, SARS-CoV-2, and animal-origin SARS-CoV-2 related viruses. Our findings demonstrated that fox ACE2 exhibits broad binding capabilities to receptor-binding domains (RBDs) of sarbecoviruses. We further determined the cryo-EM structures of fox ACE2 complexed with RBDs of SARS-CoV, SARS-CoV-2 prototype (PT), and Omicron BF.7. Through structural analysis, we identified that the K417 mutation can weaken the ability of SARS-CoV-2 sub-variants to bind to fox ACE2, thereby reducing the susceptibility of foxes to SARS-CoV-2 sub-variants. In addition, the Y498 residue in the SARS-CoV RBD plays a crucial role in forming a vital cation-π interaction with K353 in the fox ACE2 receptor. This interaction is the primary determinant for the higher affinity of the SARS-CoV RBD compared to that of the SARS-CoV-2 PT RBD. These results indicate that foxes serve as potential hosts for numerous sarbecoviruses, highlighting the critical importance of surveillance efforts. |
![]() | ![]() ![]() |
Methods | EM (single particle) |
Resolution | 2.96 - 3.47 Å |
Structure data | EMDB-38784, PDB-8xyz: EMDB-38792, PDB-8xzb: EMDB-38793, PDB-8xzd: |
Chemicals | ![]() ChemComp-ZN: |
Source |
|
![]() | VIRAL PROTEIN / fox ACE2/PT RBD / fox ACE2 SARS-CoV RBD / fox ACE2 / Omicron BF.7 |