EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures reveal the acetylation process of piccolo NuA4.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 122, Issue 12, Page e2414490122, Year 2025
掲載日	2025年3月25日
著者	Lin Wang / Haonan Zhang / Qi Jia / Wenyan Li / Chenguang Yang / Lijuan Ma / Ming Li / Ying Lu / Hongtao Zhu / Ping Zhu /
PubMed 要旨	NuA4 is the only essential acetyltransferase in yeast that can catalyze the acetylation of the histones H2A, H2A.Z, and H4, thereby affecting gene transcription. However, the acetylation process of ...NuA4 is the only essential acetyltransferase in yeast that can catalyze the acetylation of the histones H2A, H2A.Z, and H4, thereby affecting gene transcription. However, the acetylation process of NuA4, such as how NuA4 acetylates H4 and H2A.Z differently, remains largely elusive. Here, using cryoelectron microscopy (cryo-EM) single particle analysis, we present seven cryo-EM structures of piccolo NuA4 (pNuA4) in complex with wild-type H2A.Z or H2A.Z-mutant-containing nucleosomes in the absence or presence of acetyl coenzyme A (Ac-CoA). We revealed that, in the absence of Ac-CoA, pNuA4 adopts multiple conformations to search for its substrates. After adding Ac-CoA, the single-molecule Förster resonance energy transfer (smFRET) and cryo-EM data indicated that pNuA4 prefers to bind H4 and undergoes a dynamic conformational change to complete the acetylation. We also obtained previously unseen structures in states associated with the acetylation of H2A.Z. These cryo-EM structures and smFRET results suggest a complex acetylation process on H4 and H2A.Z by pNuA4. The results provide a comprehensive picture of the mechanism by which pNuA4 acetylates its substrates within an H2A.Z-containing nucleosome.
リンク	Proc Natl Acad Sci U S A / PubMed:40100634 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 7.7 Å
構造データ	38021 8x2x EMDB-38021, PDB-8x2x: The piccolo NuA4 bound to the H2A.Z nucleosome complex at pre-H4-acetylation state 手法: EM (単粒子) / 解像度: 3.8 Å 38022 8x2y EMDB-38022, PDB-8x2y: The class1 of piccolo NuA4 bound to the H2A.Z nucleosome complex at harboring state 手法: EM (単粒子) / 解像度: 4.1 Å 38023 8x2z EMDB-38023, PDB-8x2z: The class2 of piccolo NuA4 bound to the H2A.Z nucleosome complex at harboring state 手法: EM (単粒子) / 解像度: 3.9 Å 38024 8x30 EMDB-38024, PDB-8x30: Structure of piccolo NuA4 and H2A.Z nucleosome 2:1 complex 手法: EM (単粒子) / 解像度: 4.3 Å 38025 8x31 EMDB-38025, PDB-8x31: The piccolo NuA4 bound to the H2A.Z nucleosome complex with Ac-CoA at resetting state 手法: EM (単粒子) / 解像度: 6.2 Å 38026 8x32 EMDB-38026, PDB-8x32: The piccolo NuA4 bound to the H2A.Z nucleosome-H4KQ Complex with Ac-CoA at resetting state 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-60587: The piccolo NuA4 bound to the H2A.Z nucleosome-H4KQ complex without Ac-CoA at pre-H2A.Z-acetylation state 手法: EM (単粒子) / 解像度: 7.7 Å
由来	saccharomyces cerevisiae (パン酵母) escherichia coli (大腸菌) schistosoma japonicum (にほんじゅうけつきゅうちゅう)
キーワード	GENE REGULATION / Nua4 / nucleosome