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TitleBacterial genome-encoded ParMs.
Journal, issue, pagesJ Biol Chem, Vol. 301, Issue 7, Page 110351, Year 2025
Publish dateJun 9, 2025
AuthorsSamson Ali / Adrian Koh / David Popp / Kotaro Tanaka / Yoshihito Kitaoku / Naoyuki Miyazaki / Kenji Iwasaki / Kaoru Mitsuoka / Robert C Robinson / Akihiro Narita /
PubMed AbstractParMs generally exist on low-copy number plasmids where they contribute to plasmid segregation and stable inheritance. We carried out bioinformatics analysis, which indicated that ParM genes are not ...ParMs generally exist on low-copy number plasmids where they contribute to plasmid segregation and stable inheritance. We carried out bioinformatics analysis, which indicated that ParM genes are not only confined to plasmids but are also occasionally found on genomes. Here we report the discovery and characterization of two chromosome-encoded ParMs (cParMs) from the genomes of Desulfitobacterium hafniense (Dh-cParM1) and Clostridium botulinum (Cb-cParM). Both cParMs form filaments, exhibit nucleotide hydrolysis, and possess characteristic ParM subunit structures. Dh-cParM1 forms single and tightly coupled double filaments and is highly conserved on the chromosomes of five of six Desulfitobacterium species. Interestingly, these bacteria have not been reported to harbor plasmids. Cb-cParM possesses unique properties. Its filaments were stable after nucleotide hydrolysis and Pi release, and its ParR (Cb-cParR) did not affect the initial phase of Cb-cParM polymerization but displayed properties of a depolymerization factor for mature filaments. These results indicate functional, polymerizing ParMs can be encoded on genomes, suggesting that ParM roles may extend to other functions beyond plasmid segregation.
External linksJ Biol Chem / PubMed:40499760 / PubMed Central
MethodsEM (helical sym.)
Resolution3.5 - 8.6 Å
Structure data

33007

7x54

EMDB-33007, PDB-7x54:
A Cbc-ParM filament with ADP
Method: EM (helical sym.) / Resolution: 3.9 Å

33008

7x55

EMDB-33008, PDB-7x55:
A Cbc-ParM filament with GTP and a short incubation time
Method: EM (helical sym.) / Resolution: 8.6 Å

33009

7x56

EMDB-33009, PDB-7x56:
A Cbc-ParM filament with GDP
Method: EM (helical sym.) / Resolution: 3.5 Å

33012

7x59

EMDB-33012, PDB-7x59:
A Cbc-ParM filament with GTP or GDPPi
Method: EM (helical sym.) / Resolution: 6.5 Å

37996

8x1i

EMDB-37996: ParM present of genome of Desufitobacterium hafniense - Dc-cParM1
PDB-8x1i: ParM present of genome of Desufitobacterium hafniense - Dh-cParM1
Method: EM (helical sym.) / Resolution: 4.0 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

Source
  • clostridium botulinum (bacteria)
  • clostridium botulinum bf (bacteria)
  • desulfitobacterium hafniense y51 (bacteria)
KeywordsCELL CYCLE / ParM actin-like plasmid segregation / STRUCTURAL PROTEIN / ParM / genome / segregation / plasmid

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