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-Structure paper
| タイトル | Bacterial genome-encoded ParMs. |
|---|---|
| ジャーナル・号・ページ | J Biol Chem, Vol. 301, Issue 7, Page 110351, Year 2025 |
| 掲載日 | 2025年6月9日 |
著者 | Samson Ali / Adrian Koh / David Popp / Kotaro Tanaka / Yoshihito Kitaoku / Naoyuki Miyazaki / Kenji Iwasaki / Kaoru Mitsuoka / Robert C Robinson / Akihiro Narita / ![]() |
| PubMed 要旨 | ParMs generally exist on low-copy number plasmids where they contribute to plasmid segregation and stable inheritance. We carried out bioinformatics analysis, which indicated that ParM genes are not ...ParMs generally exist on low-copy number plasmids where they contribute to plasmid segregation and stable inheritance. We carried out bioinformatics analysis, which indicated that ParM genes are not only confined to plasmids but are also occasionally found on genomes. Here we report the discovery and characterization of two chromosome-encoded ParMs (cParMs) from the genomes of Desulfitobacterium hafniense (Dh-cParM1) and Clostridium botulinum (Cb-cParM). Both cParMs form filaments, exhibit nucleotide hydrolysis, and possess characteristic ParM subunit structures. Dh-cParM1 forms single and tightly coupled double filaments and is highly conserved on the chromosomes of five of six Desulfitobacterium species. Interestingly, these bacteria have not been reported to harbor plasmids. Cb-cParM possesses unique properties. Its filaments were stable after nucleotide hydrolysis and Pi release, and its ParR (Cb-cParR) did not affect the initial phase of Cb-cParM polymerization but displayed properties of a depolymerization factor for mature filaments. These results indicate functional, polymerizing ParMs can be encoded on genomes, suggesting that ParM roles may extend to other functions beyond plasmid segregation. |
リンク | J Biol Chem / PubMed:40499760 / PubMed Central |
| 手法 | EM (らせん対称) |
| 解像度 | 3.5 - 8.6 Å |
| 構造データ | EMDB-33007, PDB-7x54: EMDB-33008, PDB-7x55: EMDB-33009, PDB-7x56: EMDB-33012, PDB-7x59: EMDB-37996: ParM present of genome of Desufitobacterium hafniense - Dc-cParM1 |
| 化合物 | ![]() ChemComp-ADP: ![]() ChemComp-MG: ![]() ChemComp-GTP: ![]() ChemComp-GDP: |
| 由来 |
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キーワード | CELL CYCLE / ParM actin-like plasmid segregation / STRUCTURAL PROTEIN / ParM / genome / segregation / plasmid |
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