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TitleEnhancing the Imine Reductase Activity of a Promiscuous Glucose Dehydrogenase for Scalable Manufacturing of a Chiral Neprilysin Inhibitor Precursor
Journal, issue, pagesAcs Catalysis, Vol. 14, Page 7087-7096, Year 2024
Publish dateFeb 13, 2024 (structure data deposition date)
AuthorsYi, X. / Kleinbeck, F. / Ching, C. / Boghospor, L. / Gomes, S. / Alvizo, O. / Allmendinger, T. / Fell, J. / Subramanian, N. / Li, M. ...Yi, X. / Kleinbeck, F. / Ching, C. / Boghospor, L. / Gomes, S. / Alvizo, O. / Allmendinger, T. / Fell, J. / Subramanian, N. / Li, M. / Garcia, R. / Riggins, J. / Entwistle, D. / Richter, Y. / Gschwend, D. / Lauener, L. / Peat, T.S. / Lebhar, H. / Schlama, T. / Ruch, T.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.37 - 1.66 Å
Structure data

PDB-8w0n:
IRED crystal structure
Method: X-RAY DIFFRACTION / Resolution: 1.37 Å

PDB-8w0o:
GDH-105 crystal structure
Method: X-RAY DIFFRACTION / Resolution: 1.66 Å

Chemicals

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

ChemComp-DMS:
DIMETHYL SULFOXIDE / DMSO, precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

Source
  • bacillus subtilis (bacteria)
KeywordsOXIDOREDUCTASE / protein engineering / imine reductase / scalable manufacturing / glucose dehydrogenase

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