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| タイトル | Antibody-Based Affinity Cryoelectron Microscopy at 2.6-Å Resolution. |
|---|---|
| ジャーナル・号・ページ | Structure, Vol. 24, Issue 11, Page 1984-1990, Year 2016 |
| 掲載日 | 2016年11月1日 |
著者 | Guimei Yu / Kunpeng Li / Pengwei Huang / Xi Jiang / Wen Jiang / ![]() |
| PubMed 要旨 | The affinity cryoelectron microscopy (cryo-EM) approach has been explored in recent years to simplify and/or improve the sample preparation for cryo-EM, which can bring previously challenging ...The affinity cryoelectron microscopy (cryo-EM) approach has been explored in recent years to simplify and/or improve the sample preparation for cryo-EM, which can bring previously challenging specimens such as those of low abundance and/or unpurified ones within reach of the cryo-EM technique. Despite the demonstrated successes for solving structures to low to intermediate resolutions, the lack of near-atomic structures using this approach has led to a common perception of affinity cryo-EM as a niche technique incapable of reaching high resolutions. Here, we report a ∼2.6-Å structure solved using the antibody-based affinity grid approach with low-concentration Tulane virus purified from a low-yield cell-culture system that has been challenging to standard cryo-EM grid preparation. Quantitative analyses of the structure indicate data and reconstruction quality comparable with the conventional grid preparation method using samples at high concentration. |
リンク | Structure / PubMed:27806259 / PubMed Central |
| 手法 | EM (単粒子) |
| 解像度 | 2.6 Å |
| 構造データ | |
| 由来 |
|
キーワード | VIRUS / Tualne virus / Calicivirus / norovirus |
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万見文献について



著者
リンク

tulane virus (ウイルス)
キーワード