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Structure paper

TitlePseudomonas effector AvrB is a glycosyltransferase that rhamnosylates plant guardee protein RIN4.
Journal, issue, pagesSci Adv, Vol. 10, Page eadd5108-eadd5108, Year 2024
Publish dateAug 21, 2023 (structure data deposition date)
AuthorsPeng, W. / Garcia, N. / Servage, K.A. / Kohler, J.J. / Ready, J.M. / Tomchick, D.R. / Fernandez, J. / Orth, K.
External linksSci Adv / PubMed:38354245
MethodsX-ray diffraction
Resolution1.29 - 2.85 Å
Structure data

PDB-8twj:
AvrB_R266A bound with UDP
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-8two:
AvrB bound with UDP and RIN4_T166-Rha
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-8tws:
AvrB bound with UDP-rhamnose and RIN4 C-NOI motif
Method: X-RAY DIFFRACTION / Resolution: 2.85 Å

PDB-8txf:
AvrB bound with RIN4 C-NOI motif
Method: X-RAY DIFFRACTION / Resolution: 1.29 Å

Chemicals

ChemComp-UDP:
URIDINE-5'-DIPHOSPHATE / UDP*YM

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-HOH:
WATER

ChemComp-RAM:
alpha-L-rhamnopyranose

ChemComp-AWU:
[[(2~{R},3~{S},4~{R},5~{R})-5-[2,4-bis(oxidanylidene)pyrimidin-1-yl]-3,4-bis(oxidanyl)oxolan-2-yl]methoxy-oxidanyl-phosphoryl] [(2~{R},3~{R},4~{R},5~{R},6~{S})-6-methyl-3,4,5-tris(oxidanyl)oxan-2-yl] hydrogen phosphate

Source
  • pseudomonas syringae (bacteria)
  • arabidopsis thaliana (thale cress)
KeywordsTRANSFERASE / Protein complex

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