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TitleMolecular basis of Gabija anti-phage supramolecular assemblies.
Journal, issue, pagesNat Struct Mol Biol, Vol. 31, Issue 8, Page 1243-1250, Year 2024
Publish dateApr 16, 2024
AuthorsXiao-Yuan Yang / Zhangfei Shen / Jiale Xie / Jacelyn Greenwald / Ila Marathe / Qingpeng Lin / Wen Jun Xie / Vicki H Wysocki / Tian-Min Fu /
PubMed AbstractAs one of the most prevalent anti-phage defense systems in prokaryotes, Gabija consists of a Gabija protein A (GajA) and a Gabija protein B (GajB). The assembly and function of the Gabija system ...As one of the most prevalent anti-phage defense systems in prokaryotes, Gabija consists of a Gabija protein A (GajA) and a Gabija protein B (GajB). The assembly and function of the Gabija system remain unclear. Here we present cryo-EM structures of Bacillus cereus GajA and GajAB complex, revealing tetrameric and octameric assemblies, respectively. In the center of the complex, GajA assembles into a tetramer, which recruits two sets of GajB dimer at opposite sides of the complex, resulting in a 4:4 GajAB supramolecular complex for anti-phage defense. Further biochemical analysis showed that GajA alone is sufficient to cut double-stranded DNA and plasmid DNA, which can be inhibited by ATP. Unexpectedly, the GajAB displays enhanced activity for plasmid DNA, suggesting a role of substrate selection by GajB. Together, our study defines a framework for understanding anti-phage immune defense by the GajAB complex.
External linksNat Struct Mol Biol / PubMed:38627580 / PubMed Central
MethodsEM (single particle)
Resolution2.79 - 3.23 Å
Structure data

41314

8tjy

EMDB-41314, PDB-8tjy:
Structure of Gabija AB complex
Method: EM (single particle) / Resolution: 2.79 Å

41319

8tk0

EMDB-41319, PDB-8tk0:
Structure of Gabija AB complex
Method: EM (single particle) / Resolution: 3.23 Å

41321

8tk1

EMDB-41321, PDB-8tk1:
Structure of Gabija AB complex 1
Method: EM (single particle) / Resolution: 2.98 Å

Source
  • bacillus cereus (bacteria)
KeywordsIMMUNE SYSTEM / Anti-phage defense / Tetramer / DNA recognition and cleavage / Viral infection / Bacterial immune system

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