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Structure paper

TitleInhibition mechanism of NKCC1 involves the carboxyl terminus and long-range conformational coupling.
Journal, issue, pagesSci Adv, Vol. 8, Issue 43, Page eabq0952, Year 2022
Publish dateOct 28, 2022
AuthorsMitchell A Moseng / Chih-Chia Su / Kerri Rios / Meng Cui / Meinan Lyu / Przemyslaw Glaza / Philip A Klenotic / Eric Delpire / Edward W Yu /
PubMed AbstractThe Na-K-2Cl cotransporter-1 (NKCC1) is an electroneutral Na-dependent transporter responsible for simultaneously translocating Na, K, and Cl ions into cells. In human tissue, NKCC1 plays a critical ...The Na-K-2Cl cotransporter-1 (NKCC1) is an electroneutral Na-dependent transporter responsible for simultaneously translocating Na, K, and Cl ions into cells. In human tissue, NKCC1 plays a critical role in regulating cytoplasmic volume, fluid intake, chloride homeostasis, and cell polarity. Here, we report four structures of human NKCC1 (hNKCC1), both in the absence and presence of loop diuretic (bumetanide or furosemide), using single-particle cryo-electron microscopy. These structures allow us to directly observe various novel conformations of the hNKCC1 dimer. They also reveal two drug-binding sites located at the transmembrane and cytosolic carboxyl-terminal domains, respectively. Together, our findings enable us to delineate an inhibition mechanism that involves a coupled movement between the cytosolic and transmembrane domains of hNKCC1.
External linksSci Adv / PubMed:36306358 / PubMed Central
MethodsEM (single particle)
Resolution3.28 - 3.87 Å
Structure data

24074

7mxo

EMDB-24074, PDB-7mxo:
CryoEM structure of human NKCC1
Method: EM (single particle) / Resolution: 3.47 Å

24141

7n3n

EMDB-24141, PDB-7n3n:
CryoEM structure of human NKCC1 state Fu-I
Method: EM (single particle) / Resolution: 3.33 Å

25092

7sfl

EMDB-25092, PDB-7sfl:
Human NKCC1 state Fu-II
Method: EM (single particle) / Resolution: 3.87 Å

25204

7smp

EMDB-25204, PDB-7smp:
CryoEM structure of NKCC1 Bu-I
Method: EM (single particle) / Resolution: 3.28 Å

40759

8ste

EMDB-40759, PDB-8ste:
Cryo-EM structure of NKCC1 Fu_CTD
Method: EM (single particle) / Resolution: 3.34 Å

Chemicals

ChemComp-CL:
Unknown entry

ChemComp-K:
Unknown entry

ChemComp-FUN:
5-(AMINOSULFONYL)-4-CHLORO-2-[(2-FURYLMETHYL)AMINO]BENZOIC ACID / medication*YM

ChemComp-82U:
3-(butylamino)-4-phenoxy-5-sulfamoylbenzoic acid / medication*YM

Source
  • homo sapiens (human)
  • human (human)
KeywordsMEMBRANE PROTEIN / Sodium / potassium / chloride / co-transporter / ions / human / CTD / TMD / full-length / furosemide / diuretic / loop diuretic / NKCC1 / Transport Protein / cotransporter / dimer / ion

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