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TitleActivation of plant immunity through conversion of a helper NLR homodimer into a resistosome.
Journal, issue, pagesPLoS Biol, Vol. 22, Issue 10, Page e3002868, Year 2024
Publish dateOct 18, 2024
AuthorsMuniyandi Selvaraj / AmirAli Toghani / Hsuan Pai / Yu Sugihara / Jiorgos Kourelis / Enoch Lok Him Yuen / Tarhan Ibrahim / He Zhao / Rongrong Xie / Abbas Maqbool / Juan Carlos De la Concepcion / Mark J Banfield / Lida Derevnina / Benjamin Petre / David M Lawson / Tolga O Bozkurt / Chih-Hang Wu / Sophien Kamoun / Mauricio P Contreras /
PubMed AbstractNucleotide-binding domain and leucine-rich repeat (NLR) proteins can engage in complex interactions to detect pathogens and execute a robust immune response via downstream helper NLRs. However, the ...Nucleotide-binding domain and leucine-rich repeat (NLR) proteins can engage in complex interactions to detect pathogens and execute a robust immune response via downstream helper NLRs. However, the biochemical mechanisms of helper NLR activation by upstream sensor NLRs remain poorly understood. Here, we show that the coiled-coil helper NLR NRC2 from Nicotiana benthamiana accumulates in vivo as a homodimer that converts into a higher-order oligomer upon activation by its upstream virus disease resistance protein Rx. The cryo-EM structure of NbNRC2 in its resting state revealed intermolecular interactions that mediate homodimer formation and contribute to immune receptor autoinhibition. These dimerization interfaces have diverged between paralogous NRC proteins to insulate critical network nodes and enable redundant immune pathways, possibly to minimise undesired cross-activation and evade pathogen suppression of immunity. Our results expand the molecular mechanisms of NLR activation pointing to transition from homodimers to higher-order oligomeric resistosomes.
External linksPLoS Biol / PubMed:39423240 / PubMed Central
MethodsEM (single particle)
Resolution3.9 Å
Structure data

19121

8rfh

EMDB-19121, PDB-8rfh:
CryoEM structure of the plant helper NLR NRC2 in its resting state
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • nicotiana benthamiana (plant)
KeywordsIMMUNE SYSTEM / NLR protein / Helper NLRl / plant immunity / R-protein

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