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Structure paper

TitleCapturing the blue-light activated state of the Phot-LOV1 domain from Chlamydomonas reinhardtii using time-resolved serial synchrotron crystallography.
Journal, issue, pagesIucrj, Vol. 11, Page 792-808, Year 2024
Publish dateSep 11, 2023 (structure data deposition date)
AuthorsGotthard, G. / Mous, S. / Weinert, T. / Maia, R.N.A. / James, D. / Dworkowski, F. / Gashi, D. / Furrer, A. / Ozerov, D. / Panepucci, E. ...Gotthard, G. / Mous, S. / Weinert, T. / Maia, R.N.A. / James, D. / Dworkowski, F. / Gashi, D. / Furrer, A. / Ozerov, D. / Panepucci, E. / Wang, M. / Schertler, G.F.X. / Heberle, J. / Standfuss, J. / Nogly, P.
External linksIucrj / PubMed:39037420
MethodsX-ray diffraction
Resolution1.35 - 3.1 Å
Structure data

PDB-8qi8:
Cryogenic temperature dark state structure of CrPhotLOV1
Method: X-RAY DIFFRACTION / Resolution: 1.35 Å

PDB-8qi9:
CrPhotLOV1 dark state structure determined by serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 1.87 Å

PDB-8qia:
CrPhotLOV1 light state structure 2.5 ms (0-5 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-8qib:
CrPhotLOV1 light state structure 7.5 ms (5-10 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-8qif:
CrPhotLOV1 light state structure 12.5 ms (10-15 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.45 Å

PDB-8qig:
CrPhotLOV1 light state structure 17.5 ms (15-20 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-8qih:
CrPhotLOV1 light state structure 22.5 ms (20-25 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-8qii:
CrPhotLOV1 light state structure 27.5 ms (25-30 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-8qik:
CrPhotLOV1 light state structure 32.5 ms (30-35 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-8qil:
CrPhotLOV1 light state structure 37.5 ms (35-40 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

PDB-8qim:
CrPhotLOV1 light state structure 42.5 ms (40-45 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-8qin:
CrPhotLOV1 light state structure 47.5 ms (45-50 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-8qio:
CrPhotLOV1 light state structure 52.5 ms (50-55 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.75 Å

PDB-8qip:
CrPhotLOV1 light state structure 57.5 ms (55-60 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-8qiq:
CrPhotLOV1 light state structure 62.5 ms (60-65 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-8qir:
CrPhotLOV1 light state structure 67.5 ms (65-70 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-8qis:
CrPhotLOV1 light state structure 72.5 ms (70-75 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-8qit:
CrPhotLOV1 light state structure 77.5 ms (75-80 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 2.9 Å

PDB-8qiu:
CrPhotLOV1 light state structure 82.5 ms (80-85 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-8qiv:
CrPhotLOV1 light state structure 87.5 ms (85-90 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-8qiw:
CrPhotLOV1 light state structure 92.5 ms (90-95 ms) after illumination determined by time-resolved serial synchrotron crystallography at room temperature
Method: X-RAY DIFFRACTION / Resolution: 3.05 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-HOH:
WATER

Source
  • chlamydomonas reinhardtii (plant)
KeywordsFLAVOPROTEIN / PHOTOTROPIN / FLAVIN / ELECTRON TRANSPORT

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