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Structure paper

TitleThe structure of immature tick-borne encephalitis virus supports the collapse model of flavivirus maturation.
Journal, issue, pagesSci Adv, Vol. 10, Issue 27, Page eadl1888, Year 2024
Publish dateJul 5, 2024
AuthorsMaria Anastasina / Tibor Füzik / Aušra Domanska / Lauri Ilmari Aurelius Pulkkinen / Lenka Šmerdová / Petra Pokorná Formanová / Petra Straková / Jiří Nováček / Daniel Růžek / Pavel Plevka / Sarah Jane Butcher /
PubMed AbstractWe present structures of three immature tick-borne encephalitis virus (TBEV) isolates. Our atomic models of the major viral components, the E and prM proteins, indicate that the pr domains of prM ...We present structures of three immature tick-borne encephalitis virus (TBEV) isolates. Our atomic models of the major viral components, the E and prM proteins, indicate that the pr domains of prM have a critical role in holding the heterohexameric prM3E3 spikes in a metastable conformation. Destabilization of the prM furin-sensitive loop at acidic pH facilitates its processing. The prM topology and domain assignment in TBEV is similar to the mosquito-borne Binjari virus, but is in contrast to other immature flavivirus models. These results support that prM cleavage, the collapse of E protein ectodomains onto the virion surface, the large movement of the membrane domains of both E and M, and the release of the pr fragment from the particle render the virus mature and infectious. Our work favors the collapse model of flavivirus maturation warranting further studies of immature flaviviruses to determine the sequence of events and mechanistic details driving flavivirus maturation.
External linksSci Adv / PubMed:38959313 / PubMed Central
MethodsEM (single particle)
Resolution3.9 - 8.64 Å
Structure data

17808

8ppq

EMDB-17808, PDB-8ppq:
Tick-borne encephalitis virus Kuutsalo-14 prM3E3 trimer
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-17809: Tick-borne encephalitis virus Kuutsalo-14 immature virus particle
Method: EM (single particle) / Resolution: 7.1 Å

EMDB-17945: Tick-borne encephalitis virus (strain HYPR) immature particle
Method: EM (single particle) / Resolution: 8.64 Å

EMDB-17946: Tick-borne encephalitis virus (strain Neudoerfl) immature particle
Method: EM (single particle) / Resolution: 7.02 Å

17947

8puv

EMDB-17947, PDB-8puv:
Trimeric prM/E spike of Tick-borne encephalitis virus immature particle
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

Source
  • tick-borne encephalitis virus
KeywordsVIRUS / Complex / Spike / Fusion protein / Glycosylation / Transmembrane protein / Maturation / Trimer / Hexamer / Dimer / immature particle / tick-borne / flavivirus / spike trimer

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