+Search query
-Structure paper
Title | Cryo-EM structure of a bacteriophage M13 mini variant. |
---|---|
Journal, issue, pages | Nat Commun, Vol. 14, Issue 1, Page 5421, Year 2023 |
Publish date | Sep 5, 2023 |
Authors | Qi Jia / Ye Xiang / |
PubMed Abstract | Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM ...Filamentous bacteriophages package their circular, single stranded DNA genome with the major coat protein pVIII and the minor coat proteins pIII, pVII, pVI, and pIX. Here, we report the cryo-EM structure of a ~500 Å long bacteriophage M13 mini variant. The distal ends of the mini phage are sealed by two cap-like complexes composed of the minor coat proteins. The top cap complex consists of pVII and pIX, both exhibiting a single helix structure. Arg33 of pVII and Glu29 of pIX, located on the inner surface of the cap, play a key role in recognizing the genome packaging signal. The bottom cap complex is formed by the hook-like structures of pIII and pVI, arranged in helix barrels. Most of the inner ssDNA genome adopts a double helix structure with a similar pitch to that of the A-form double-stranded DNA. These findings provide insights into the assembly of filamentous bacteriophages. |
External links | Nat Commun / PubMed:37669979 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.1 - 3.9 Å |
Structure data | EMDB-35793, PDB-8ixj: EMDB-35794, PDB-8ixk: EMDB-35795, PDB-8ixl: EMDB-35796: Asymmetric reconstruction of the middle segment of the bacteriophage M13 mini variant EMDB-35797: Asymmetric reconstruction of bottom segment of the bacteriophage M13 mini variant EMDB-35798: Full length of the bacteriophage M13 mini variant EMDB-35805: Asymmetric reconstruction of the full length of the bacteriophage M13 variant |
Source |
|
Keywords | VIRAL PROTEIN / Viral coat protein / M13 |