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-Structure paper
タイトル | Contributions of histone tail clipping and acetylation in nucleosome transcription by RNA polymerase II. |
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ジャーナル・号・ページ | Nucleic Acids Res, Vol. 51, Issue 19, Page 10364-10374, Year 2023 |
掲載日 | 2023年10月27日 |
著者 | Takumi Oishi / Suguru Hatazawa / Tomoya Kujirai / Junko Kato / Yuki Kobayashi / Mitsuo Ogasawara / Munetaka Akatsu / Haruhiko Ehara / Shun-Ichi Sekine / Gosuke Hayashi / Yoshimasa Takizawa / Hitoshi Kurumizaka / |
PubMed 要旨 | The N-terminal tails of histones protrude from the nucleosome core and are target sites for histone modifications, such as acetylation and methylation. Histone acetylation is considered to enhance ...The N-terminal tails of histones protrude from the nucleosome core and are target sites for histone modifications, such as acetylation and methylation. Histone acetylation is considered to enhance transcription in chromatin. However, the contribution of the histone N-terminal tail to the nucleosome transcription by RNA polymerase II (RNAPII) has not been clarified. In the present study, we reconstituted nucleosomes lacking the N-terminal tail of each histone, H2A, H2B, H3 or H4, and performed RNAPII transcription assays. We found that the N-terminal tail of H3, but not H2A, H2B and H4, functions in RNAPII pausing at the SHL(-5) position of the nucleosome. Consistently, the RNAPII transcription assay also revealed that the nucleosome containing N-terminally acetylated H3 drastically alleviates RNAPII pausing at the SHL(-5) position. In addition, the H3 acetylated nucleosome produced increased amounts of the run-off transcript. These results provide important evidence that the H3 N-terminal tail plays a role in RNAPII pausing at the SHL(-5) position of the nucleosome, and its acetylation directly alleviates this nucleosome barrier. |
リンク | Nucleic Acids Res / PubMed:37718728 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.36 - 3.44 Å |
構造データ | EMDB-36389, PDB-8jl9: EMDB-36390, PDB-8jla: EMDB-36391, PDB-8jlb: EMDB-36393, PDB-8jld: |
由来 |
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キーワード | GENE REGULATION/DNA / nuclear protein / chromatin (クロマチン) / GENE REGULATION-DNA COMPLEX |