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Structure paper

TitleGolpHCat (TMEM87A), a unique voltage-dependent cation channel in Golgi apparatus, contributes to Golgi-pH maintenance and hippocampus-dependent memory.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 5830, Year 2024
Publish dateJul 11, 2024
AuthorsHyunji Kang / Ah-Reum Han / Aihua Zhang / Heejin Jeong / Wuhyun Koh / Jung Moo Lee / Hayeon Lee / Hee Young Jo / Miguel A Maria-Solano / Mridula Bhalla / Jea Kwon / Woo Suk Roh / Jimin Yang / Hyun Joo An / Sun Choi / Ho Min Kim / C Justin Lee /
PubMed AbstractImpaired ion channels regulating Golgi pH lead to structural alterations in the Golgi apparatus, such as fragmentation, which is found, along with cognitive impairment, in Alzheimer's disease. ...Impaired ion channels regulating Golgi pH lead to structural alterations in the Golgi apparatus, such as fragmentation, which is found, along with cognitive impairment, in Alzheimer's disease. However, the causal relationship between altered Golgi structure and cognitive impairment remains elusive due to the lack of understanding of ion channels in the Golgi apparatus of brain cells. Here, we identify that a transmembrane protein TMEM87A, renamed Golgi-pH-regulating cation channel (GolpHCat), expressed in astrocytes and neurons that contributes to hippocampus-dependent memory. We find that GolpHCat displays unique voltage-dependent currents, which is potently inhibited by gluconate. Additionally, we gain structural insights into the ion conduction through GolpHCat at the molecular level by determining three high-resolution cryogenic-electron microscopy structures of human GolpHCat. GolpHCat-knockout mice show fragmented Golgi morphology and altered protein glycosylation and functions in the hippocampus, leading to impaired spatial memory. These findings suggest a molecular target for Golgi-related diseases and cognitive impairment.
External linksNat Commun / PubMed:38992057 / PubMed Central
MethodsEM (single particle)
Resolution3.1 - 3.6 Å
Structure data

34998

8hsi

EMDB-34998, PDB-8hsi:
Cryo-EM structure of human TMEM87A, PE-bound
Method: EM (single particle) / Resolution: 3.1 Å

35017

8htt

EMDB-35017, PDB-8htt:
Cryo-EM structure of human TMEM87A, gluconate-bound
Method: EM (single particle) / Resolution: 3.6 Å

37069

8kb4

EMDB-37069, PDB-8kb4:
Cryo-EM structure of human TMEM87A A308M
Method: EM (single particle) / Resolution: 3.1 Å

Chemicals

ChemComp-L9Q:
(1S)-2-{[(S)-(2-aminoethoxy)(hydroxy)phosphoryl]oxy}-1-[(octadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate

ChemComp-CLR:
CHOLESTEROL

ChemComp-GCO:
D-gluconic acid

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose

ChemComp-65I:
(9R,12R)-15-amino-12-hydroxy-6,12-dioxo-7,11,13-trioxa-12lambda~5~-phosphapentadecan-9-yl undecanoate

Source
  • homo sapiens (human)
  • human adenovirus 2
  • human adenovirus c serotype 2
KeywordsMEMBRANE PROTEIN / non-selective cation channel / ion channel / Golgi-localized protein

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