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TitleStructural insights into trehalose capture and translocation by mycobacterial LpqY-SugABC.
Journal, issue, pagesStructure, Vol. 31, Issue 10, Page 1158-11165.e3, Year 2023
Publish dateOct 5, 2023
AuthorsJingxi Liang / Xiuna Yang / Tianyu Hu / Yan Gao / Qi Yang / Haitao Yang / Wei Peng / Xiaoting Zhou / Luke W Guddat / Bing Zhang / Zihe Rao / Fengjiang Liu /
PubMed AbstractThe human pathogen, Mycobacterium tuberculosis (Mtb) relies heavily on trehalose for both survival and pathogenicity. The type I ATP-binding cassette (ABC) transporter LpqY-SugABC is the only ...The human pathogen, Mycobacterium tuberculosis (Mtb) relies heavily on trehalose for both survival and pathogenicity. The type I ATP-binding cassette (ABC) transporter LpqY-SugABC is the only trehalose import pathway in Mtb. Conformational dynamics of ABC transporters is an important feature to explain how they operate, but experimental structures are determined in a static environment. Therefore, a detailed transport mechanism cannot be elucidated because there is a lack of intermediate structures. Here, we used single-particle cryo-electron microscopy (cryo-EM) to determine the structure of the Mycobacterium smegmatis (M. smegmatis) trehalose-specific importer LpqY-SugABC complex in five different conformations. These structures have been classified and reconstructed from a single cryo-EM dataset. This study allows a comprehensive understanding of the trehalose recycling mechanism in Mycobacteria and also demonstrates the potential of single-particle cryo-EM to explore the dynamic structures of other ABC transporters and molecular machines.
External linksStructure / PubMed:37619560
MethodsEM (single particle)
Resolution3.51 - 4.55 Å
Structure data

34932

8hpl

EMDB-34932, PDB-8hpl:
LpqY-SugABC in state 1
Method: EM (single particle) / Resolution: 4.29 Å

34933

8hpm

EMDB-34933, PDB-8hpm:
LpqY-SugABC in state 2
Method: EM (single particle) / Resolution: 3.82 Å

34934

8hpn

EMDB-34934, PDB-8hpn:
LpqY-SugABC in state 3
Method: EM (single particle) / Resolution: 4.55 Å

34941

8hpr

EMDB-34941, PDB-8hpr:
LpqY-SugABC in state 4
Method: EM (single particle) / Resolution: 3.75 Å

34942

8hps

EMDB-34942, PDB-8hps:
LpqY-SugABC in state 5
Method: EM (single particle) / Resolution: 3.51 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

Source
  • mycolicibacterium smegmatis mc2 155 (bacteria)
KeywordsTRANSPORT PROTEIN / Trehalose / ABC transporter / tuberculosis

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