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TitleChromophore carbonyl twisting in fluorescent biosensors encodes direct readout of protein conformations with multicolor switching.
Journal, issue, pagesCommun Chem, Vol. 6, Page 168-168, Year 2023
Publish dateJan 25, 2023 (structure data deposition date)
AuthorsAllert, M.J. / Kumar, S. / Wang, Y. / Beese, L.S. / Hellinga, H.W.
External linksCommun Chem / PubMed:37598249
MethodsX-ray diffraction
Resolution1.53 - 1.59 Å
Structure data

PDB-8fxt:
Escherichia coli periplasmic Glucose-Binding Protein glucose complex: Acrylodan conjugate attached at W183C
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-8fxu:
Thermoanaerobacter thermosaccharolyticum periplasmic Glucose-Binding Protein glucose complex: Badan conjugate attached at F17C
Method: X-RAY DIFFRACTION / Resolution: 1.59 Å

Chemicals

ChemComp-YDM:
1-[6-(dimethylamino)naphthalen-2-yl]propan-1-one

ChemComp-GLC:
alpha-D-glucopyranose

ChemComp-BGC:
beta-D-glucopyranose

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-K:
Unknown entry

ChemComp-YDQ:
2-bromo-1-[6-(dimethylamino)naphthalen-2-yl]ethan-1-one

ChemComp-CL:
Unknown entry

Source
  • escherichia coli (E. coli)
  • thermoanaerobacterium thermosaccharolyticum (bacteria)
KeywordsSUGAR BINDING PROTEIN / Periplasmic binding protein / Biosensor Fluorescent conjugate

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