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Structure paper

TitleActivation of CBASS Cap5 endonuclease immune effector by cyclic nucleotides.
Journal, issue, pagesNat. Struct. Mol. Biol., Vol. 31, Page 767-776, Year 2024
Publish dateDec 22, 2022 (structure data deposition date)
AuthorsRechkoblit, O. / Sciaky, D. / Kreitler, D.F. / Buku, A. / Kottur, J. / Aggarwal, A.K.
External linksNat. Struct. Mol. Biol. / PubMed:38321146
MethodsX-ray diffraction
Resolution1.79 - 3.16 Å
Structure data

PDB-8fm1:
Structure of CBASS Cap5 from Pseudomonas syringae in the absence of a ligand (apo form dimer)
Method: X-RAY DIFFRACTION / Resolution: 3.16 Å

PDB-8fmf:
Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-diAMP ligand (1 tetramer in the AU)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-8fmg:
Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-diAMP ligand (3 tetramers in the AU)
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

PDB-8fmh:
Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-dGAMP ligand (2 tetramers in the AU)
Method: X-RAY DIFFRACTION / Resolution: 1.87 Å

Chemicals

ChemComp-ZN:
Unknown entry


ChemComp, No image

ChemComp-Y4F:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-RWB:
dodecaethylene glycol monomethyl ether

ChemComp-MG:
Unknown entry

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-HOH:
WATER

ChemComp-4UR:
3'2'-cGAMP

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • pseudomonas syringae (bacteria)
KeywordsIMMUNE SYSTEM / cyclic dinucleotide / bacterial immunity / CBASS / Cap5 effector DNA endonuclease / viral defense

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