Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular mechanism for Tn7-like transposon recruitment by a type I-B CRISPR effector.
Journal, issue, pages	Cell, Vol. 186, Issue 19, Page 4204-4215.e19, Year 2023
Publish date	Sep 14, 2023
Authors	Shukun Wang / Clinton Gabel / Romana Siddique / Thomas Klose / Leifu Chang /
PubMed Abstract	Tn7-like transposons have co-opted CRISPR-Cas systems to facilitate the movement of their own DNA. These CRISPR-associated transposons (CASTs) are promising tools for programmable gene knockin. A key ...Tn7-like transposons have co-opted CRISPR-Cas systems to facilitate the movement of their own DNA. These CRISPR-associated transposons (CASTs) are promising tools for programmable gene knockin. A key feature of CASTs is their ability to recruit Tn7-like transposons to nuclease-deficient CRISPR effectors. However, how Tn7-like transposons are recruited by diverse CRISPR effectors remains poorly understood. Here, we present the cryo-EM structure of a recruitment complex comprising the Cascade complex, TniQ, TnsC, and the target DNA in the type I-B CAST from Peltigera membranacea cyanobiont 210A. Target DNA recognition by Cascade induces conformational changes in Cas6 and primes TniQ recruitment through its C-terminal domain. The N-terminal domain of TniQ is bound to the seam region of the TnsC spiral heptamer. Our findings provide insights into the diverse mechanisms for the recruitment of Tn7-like transposons to CRISPR effectors and will aid in the development of CASTs as gene knockin tools.
External links	Cell / PubMed:37557170 / PubMed Central
Methods	EM (single particle)
Resolution	2.83 - 3.65 Å
Structure data	28980 8fcj EMDB-28980, PDB-8fcj: Cryo-EM structure of Cascade-DNA (P23) complex in type I-B CAST system Method: EM (single particle) / Resolution: 2.83 Å 28993 8fcu EMDB-28993, PDB-8fcu: Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex in type I-B CAST system Method: EM (single particle) / Resolution: 3.19 Å 28994 8fcv EMDB-28994, PDB-8fcv: Cryo-EM structure of TnsC-TniQ-DNA complex in type I-B CAST system Method: EM (single particle) / Resolution: 2.95 Å 28997 8fcw EMDB-28997, PDB-8fcw: Cryo-EM structure of TnsC-DNA complex in type I-B CAST system Method: EM (single particle) / Resolution: 2.87 Å 28998 8fcx EMDB-28998, PDB-8fcx: Cryo-EM structure of TnsC oligomer in type I-B CAST system Method: EM (single particle) / Resolution: 3.04 Å 29000 8fd2 EMDB-29000, PDB-8fd2: Cryo-EM structure of Cascade complex in type I-B CAST system Method: EM (single particle) / Resolution: 3.65 Å 29001 8fd3 EMDB-29001, PDB-8fd3: Cryo-EM structure of Cascade-PAM complex in type I-B CAST system Method: EM (single particle) / Resolution: 3.12 Å 29039 8ff4 EMDB-29039, PDB-8ff4: Cryo-EM structure of Cascade-DNA-TniQ-TnsC complex (composite) in type I-B CAST system Method: EM (single particle) / Resolution: 3.6 Å 29040 8ff5 EMDB-29040, PDB-8ff5: Cryo-EM structure of Cascade-DNA-fullRloop in type I-B CAST system Method: EM (single particle) / Resolution: 3.13 Å
Chemicals	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-MG*: Unknown entry
Source	peltigera membranacea (fungus) nostoc sp. 'peltigera membranacea cyanobiont' 210a (bacteria) synthetic construct (others)
Keywords	DNA BINDING PROTEIN / CRISPR / DNA recognition / TnsC / TniQ / Transposon / DNA / CAST / Cascade / I-B