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Structure paper

TitleLight controlled reversible Michael addition of cysteine: a new tool for dynamic site-specific labeling of proteins.
Journal, issue, pagesAnalyst, Vol. 148, Page 1085-1092, Year 2023
Publish dateJun 6, 2022 (structure data deposition date)
AuthorsMaity, S. / Bingham, C. / Sheng, W. / Ehyaei, N. / Chakraborty, D. / Tahmasebi-Nick, S. / Kimmel, T.E. / Vasileiou, C. / Geiger, J.H. / Borhan, B.
External linksAnalyst / PubMed:36722993
MethodsX-ray diffraction
Resolution1.32 - 1.69 Å
Structure data

PDB-8d6h:
Q108K:K40L:T51C:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V after UV irradiation
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-8d6l:
Q108K:K40L:T51C:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V
Method: X-RAY DIFFRACTION / Resolution: 1.69 Å

PDB-8d6n:
Q108K:K40L:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V
Method: X-RAY DIFFRACTION / Resolution: 1.42 Å

PDB-8db2:
Q108K:K40L:T51C:T53A:R58L:Q38F mutant of hCRBPII bound to synthetic fluorophore CM1V
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

PDB-8dn1:
Q108K:K40L:T51C:T53A:R58L:Q38F:Q4F mutant of hCRBPII bound to synthetic fluorophore CM1V at pH 7.2
Method: X-RAY DIFFRACTION / Resolution: 1.32 Å

Chemicals

ChemComp-GOL:
GLYCEROL

ChemComp-ACT:
ACETATE ION

ChemComp-RH6:
(2E)-3-[7-(diethylamino)-2-oxo-2H-1-benzopyran-3-yl]prop-2-enal, bound form

ChemComp-HOH:
WATER

ChemComp-MLA:
MALONIC ACID

Source
  • homo sapiens (human)
KeywordsTRANSPORT PROTEIN / hCRBPII / cellular retinol binding protein II / covalently bound fluorophore

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