Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Symport and antiport mechanisms of human glutamate transporters.
Journal, issue, pages	Nat Commun, Vol. 14, Issue 1, Page 2579, Year 2023
Publish date	May 4, 2023
Authors	Biao Qiu / Olga Boudker /
PubMed Abstract	Excitatory amino acid transporters (EAATs) uptake glutamate into glial cells and neurons. EAATs achieve million-fold transmitter gradients by symporting it with three sodium ions and a proton, and ...Excitatory amino acid transporters (EAATs) uptake glutamate into glial cells and neurons. EAATs achieve million-fold transmitter gradients by symporting it with three sodium ions and a proton, and countertransporting a potassium ion via an elevator mechanism. Despite the availability of structures, the symport and antiport mechanisms still need to be clarified. We report high-resolution cryo-EM structures of human EAAT3 bound to the neurotransmitter glutamate with symported ions, potassium ions, sodium ions alone, or without ligands. We show that an evolutionarily conserved occluded translocation intermediate has a dramatically higher affinity for the neurotransmitter and the countertransported potassium ion than outward- or inward-facing transporters and plays a crucial role in ion coupling. We propose a comprehensive ion coupling mechanism involving a choreographed interplay between bound solutes, conformations of conserved amino acid motifs, and movements of the gating hairpin and the substrate-binding domain.
External links	Nat Commun / PubMed:37142617 / PubMed Central
Methods	EM (single particle)
Resolution	2.44 - 3.42 Å
Structure data	26985 8ctc EMDB-26985, PDB-8ctc: Human excitatory amino acid transporter 3 (EAAT3) with bound glutamate in an intermediate outward facing state Method: EM (single particle) / Resolution: 2.8 Å 26986 8ctd EMDB-26986, PDB-8ctd: Human excitatory amino acid transporter 3 (EAAT3) protomer with bound glutamate in an outward facing state Method: EM (single particle) / Resolution: 3.42 Å 26997 8cua EMDB-26997, PDB-8cua: Human excitatory amino acid transporter 3 (EAAT3) with bound potassium in an intermediate outward facing state Method: EM (single particle) / Resolution: 2.44 Å 26998 8cud EMDB-26998, PDB-8cud: Human excitatory amino acid transporter 3 (EAAT3) protomer in an outward facing apo state in 300 mM KCl Method: EM (single particle) / Resolution: 2.94 Å 27000 8cui EMDB-27000, PDB-8cui: Human excitatory amino acid transporter 3 (EAAT3) in an intermediate outward facing apo state Method: EM (single particle) / Resolution: 2.55 Å 27001 8cuj EMDB-27001, PDB-8cuj: Human excitatory amino acid transporter 3 (EAAT3) protomer in an outward facing apo state in 150 mM NMDG-Cl Method: EM (single particle) / Resolution: 3.04 Å 27006 8cv2 EMDB-27006, PDB-8cv2: Human excitatory amino acid transporter 3 (EAAT3) in an outward facing sodium-bound state Method: EM (single particle) / Resolution: 2.44 Å 27007 8cv3 EMDB-27007, PDB-8cv3: Human Excitatory excitatory amino acid transporter 3 (EAAT3) protomer at in an intermediate outward facing sodium-bound state Method: EM (single particle) / Resolution: 3.04 Å
Chemicals	ChemComp-GLU: GLUTAMIC ACID ChemComp-NA: Unknown entry ChemComp-HG: Unknown entry ChemComp-HOH: WATER ChemComp-K: Unknown entry
Source	homo sapiens (human)
Keywords	TRANSPORT PROTEIN