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Structure paper

TitlePlastic degradation by insect hexamerins: Near-atomic resolution structures of the polyethylene-degrading proteins from the wax worm saliva.
Journal, issue, pagesSci Adv, Vol. 9, Issue 38, Page eadi6813, Year 2023
Publish dateSep 22, 2023
AuthorsMercedes Spínola-Amilibia / Ramiro Illanes-Vicioso / Elena Ruiz-López / Pere Colomer-Vidal / Francisco Rodriguez-Ventura / Rosa Peces Pérez / Clemente F Arias / Tomas Torroba / Maria Solà / Ernesto Arias-Palomo / Federica Bertocchini /
PubMed AbstractPlastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran larvae is capable of oxidizing and depolymerizing polyethylene in hours at room ...Plastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran larvae is capable of oxidizing and depolymerizing polyethylene in hours at room temperature. Here, we analyze by cryo-electron microscopy (cryo-EM) 's saliva directly from the native source. The three-dimensional reconstructions reveal that the buccal secretion is mainly composed of four hexamerins belonging to the hemocyanin/phenoloxidase family, renamed Demetra, Cibeles, Ceres, and a previously unidentified factor termed Cora. Functional assays show that this factor, as its counterparts Demetra and Ceres, is also able to oxidize and degrade polyethylene. The cryo-EM data and the x-ray analysis from purified fractions show that they self-assemble primarily into three macromolecular complexes with striking structural differences that likely modulate their activity. Overall, these results establish the ground to further explore the hexamerins' functionalities, their role in vivo, and their eventual biotechnological application.
External linksSci Adv / PubMed:37729416 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution1.93 - 2.85 Å
Structure data

16521

8ca9

EMDB-16521, PDB-8ca9:
Cryo-EM structure of the Cibeles-Demetra 3:3 heterocomplex from Galleria mellonella saliva
Method: EM (single particle) / Resolution: 2.29 Å

16524

8cad

EMDB-16524, PDB-8cad:
Cryo-EM structure of the Ceres homohexamer from Galleria mellonella saliva
Method: EM (single particle) / Resolution: 2.85 Å

16531

8can

EMDB-16531, PDB-8can:
Cryo-EM structure of the Cora homohexamer from Galleria mellonella saliva
Method: EM (single particle) / Resolution: 1.93 Å

PDB-8po9:
Polyethylene oxidation hexamerin PEase Cibeles (XP_026756460) from Galleria mellonella
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-CU:
COPPER (II) ION

ChemComp-HOH:
WATER

ChemComp-TRP:
TRYPTOPHAN

ChemComp-NA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-GOL:
GLYCEROL

ChemComp-GLY:
GLYCINE

ChemComp-SO4:
SULFATE ION

Source
  • galleria mellonella (greater wax moth)
KeywordsUNKNOWN FUNCTION / Galleria mellonella / Wax worm saliva / Plastic degradation / Polyethylene degradation / PEases / Hexamerin / Hemocyanin/phenoloxidase superfamily / Metal binding / Arylphorin / OXIDOREDUCTASE / Polyethylene oxidation enzyme / PEase / XP_026756460 / Cibeles

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