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-Structure paper
タイトル | Plastic degradation by insect hexamerins: Near-atomic resolution structures of the polyethylene-degrading proteins from the wax worm saliva. |
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ジャーナル・号・ページ | Sci Adv, Vol. 9, Issue 38, Page eadi6813, Year 2023 |
掲載日 | 2023年9月22日 |
著者 | Mercedes Spínola-Amilibia / Ramiro Illanes-Vicioso / Elena Ruiz-López / Pere Colomer-Vidal / Francisco Rodriguez-Ventura / Rosa Peces Pérez / Clemente F Arias / Tomas Torroba / Maria Solà / Ernesto Arias-Palomo / Federica Bertocchini / |
PubMed 要旨 | Plastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran larvae is capable of oxidizing and depolymerizing polyethylene in hours at room ...Plastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran larvae is capable of oxidizing and depolymerizing polyethylene in hours at room temperature. Here, we analyze by cryo-electron microscopy (cryo-EM) 's saliva directly from the native source. The three-dimensional reconstructions reveal that the buccal secretion is mainly composed of four hexamerins belonging to the hemocyanin/phenoloxidase family, renamed Demetra, Cibeles, Ceres, and a previously unidentified factor termed Cora. Functional assays show that this factor, as its counterparts Demetra and Ceres, is also able to oxidize and degrade polyethylene. The cryo-EM data and the x-ray analysis from purified fractions show that they self-assemble primarily into three macromolecular complexes with striking structural differences that likely modulate their activity. Overall, these results establish the ground to further explore the hexamerins' functionalities, their role in vivo, and their eventual biotechnological application. |
リンク | Sci Adv / PubMed:37729416 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.93 - 2.85 Å |
構造データ | EMDB-16521, PDB-8ca9: EMDB-16524, PDB-8cad: EMDB-16531, PDB-8can: PDB-8po9: |
化合物 | ChemComp-CU: ChemComp-HOH: ChemComp-TRP: ChemComp-NA: ChemComp-MG: ChemComp-CL: ChemComp-MES: ChemComp-MPD: ChemComp-GOL: ChemComp-GLY: ChemComp-SO4: |
由来 |
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キーワード | UNKNOWN FUNCTION / Galleria mellonella / Wax worm saliva / Plastic degradation / Polyethylene degradation / PEases / Hexamerin / Hemocyanin/phenoloxidase superfamily / Metal binding / Arylphorin / OXIDOREDUCTASE / Polyethylene oxidation enzyme / PEase / XP_026756460 / Cibeles |