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TitleA bacterial tungsten-containing aldehyde oxidoreductase forms an enzymatic decorated protein nanowire.
Journal, issue, pagesSci Adv, Vol. 9, Issue 22, Page eadg6689, Year 2023
Publish dateJun 2, 2023
AuthorsAgnieszka Winiarska / Fidel Ramírez-Amador / Dominik Hege / Yvonne Gemmecker / Simone Prinz / Georg Hochberg / Johann Heider / Maciej Szaleniec / Jan Michael Schuller /
PubMed AbstractAldehyde oxidoreductases (AORs) are tungsten enzymes catalyzing the oxidation of many different aldehydes to the corresponding carboxylic acids. In contrast to other known AORs, the enzyme from the ...Aldehyde oxidoreductases (AORs) are tungsten enzymes catalyzing the oxidation of many different aldehydes to the corresponding carboxylic acids. In contrast to other known AORs, the enzyme from the denitrifying betaproteobacterium (AOR) consists of three different subunits (AorABC) and uses nicotinamide adenine dinucleotide (NAD) as an electron acceptor. Here, we reveal that the enzyme forms filaments of repeating AorAB protomers that are capped by a single NAD-binding AorC subunit, based on solving its structure via cryo-electron microscopy. The polyferredoxin-like subunit AorA oligomerizes to an electron-conducting nanowire that is decorated with enzymatically active and W-cofactor (W-co) containing AorB subunits. Our structure further reveals the binding mode of the native substrate benzoate in the AorB active site. This, together with quantum mechanics:molecular mechanics (QM:MM)-based modeling for the coordination of the W-co, enables formulation of a hypothetical catalytic mechanism that paves the way to further engineering for applications in synthetic biology and biotechnology.
External linksSci Adv / PubMed:37267359 / PubMed Central
MethodsEM (single particle)
Resolution3.22 Å
Structure data

16376

8c0z

EMDB-16376, PDB-8c0z:
CryoEM structure of a tungsten-containing aldehyde oxidoreductase from Aromatoleum aromaticum
Method: EM (single particle) / Resolution: 3.22 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-T7R:
tungsten cofactor

ChemComp-MG:
Unknown entry

ChemComp-BEZ:
BENZOIC ACID

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

Source
  • aromatoleum aromaticum (bacteria)
KeywordsOXIDOREDUCTASE / Tungsten-containing enzyme / nanowires.

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