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TitleStructure and mechanism of the K/H exchanger KefC.
Journal, issue, pagesNat Commun, Vol. 15, Issue 1, Page 4751, Year 2024
Publish dateJun 4, 2024
AuthorsAshutosh Gulati / Surabhi Kokane / Annemarie Perez-Boerema / Claudia Alleva / Pascal F Meier / Rei Matsuoka / David Drew /
PubMed AbstractIntracellular potassium (K) homeostasis is fundamental to cell viability. In addition to channels, K levels are maintained by various ion transporters. One major family is the proton-driven K efflux ...Intracellular potassium (K) homeostasis is fundamental to cell viability. In addition to channels, K levels are maintained by various ion transporters. One major family is the proton-driven K efflux transporters, which in gram-negative bacteria is important for detoxification and in plants is critical for efficient photosynthesis and growth. Despite their importance, the structure and molecular basis for K-selectivity is poorly understood. Here, we report ~3.1 Å resolution cryo-EM structures of the Escherichia coli glutathione (GSH)-gated K efflux transporter KefC in complex with AMP, AMP/GSH and an ion-binding variant. KefC forms a homodimer similar to the inward-facing conformation of Na/H antiporter NapA. By structural assignment of a coordinated K ion, MD simulations, and SSM-based electrophysiology, we demonstrate how ion-binding in KefC is adapted for binding a dehydrated K ion. KefC harbors C-terminal regulator of K conductance (RCK) domains, as present in some bacterial K-ion channels. The domain-swapped helices in the RCK domains bind AMP and GSH and they inhibit transport by directly interacting with the ion-transporter module. Taken together, we propose that KefC is activated by detachment of the RCK domains and that ion selectivity exploits the biophysical properties likewise adapted by K-ion-channels.
External linksNat Commun / PubMed:38834573 / PubMed Central
MethodsEM (single particle)
Resolution3.16 - 3.18 Å
Structure data

16318

8bxg

EMDB-16318: Structure of the K/H exchanger KefC
PDB-8bxg: Structure of the K/H exchanger KefC.
Method: EM (single particle) / Resolution: 3.16 Å

16319

8by2

EMDB-16319: Structure of the K+/H+ exchanger KefC with GSH
PDB-8by2: Structure of the K+/H+ exchanger KefC with GSH.
Method: EM (single particle) / Resolution: 3.18 Å

Chemicals

ChemComp-K:
Unknown entry

ChemComp-AMP:
ADENOSINE MONOPHOSPHATE / AMP*YM

ChemComp-PGW:
(1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl / phospholipid*YM

ChemComp-GSH:
GLUTATHIONE

Source
  • escherichia coli (E. coli)
KeywordsMEMBRANE PROTEIN / potassium proton exchanger / KefC / Transporter / CPA / GSH

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