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Structure paper

TitleMolecular basis for selective uptake and elimination of organic anions in the kidney by OAT1.
Journal, issue, pagesNat Struct Mol Biol, Vol. 30, Issue 11, Page 1786-1793, Year 2023
Publish dateJul 23, 2023
AuthorsJoanne L Parker / Takafumi Kato / Gabriel Kuteyi / Oleg Sitsel / Simon Newstead /
PubMed AbstractIn mammals, the kidney plays an essential role in maintaining blood homeostasis through the selective uptake, retention or elimination of toxins, drugs and metabolites. Organic anion transporters ...In mammals, the kidney plays an essential role in maintaining blood homeostasis through the selective uptake, retention or elimination of toxins, drugs and metabolites. Organic anion transporters (OATs) are responsible for the recognition of metabolites and toxins in the nephron and their eventual urinary excretion. Inhibition of OATs is used therapeutically to improve drug efficacy and reduce nephrotoxicity. The founding member of the renal organic anion transporter family, OAT1 (also known as SLC22A6), uses the export of α-ketoglutarate (α-KG), a key intermediate in the Krebs cycle, to drive selective transport and is allosterically regulated by intracellular chloride. However, the mechanisms linking metabolite cycling, drug transport and intracellular chloride remain obscure. Here, we present cryogenic-electron microscopy structures of OAT1 bound to α-KG, the antiviral tenofovir and clinical inhibitor probenecid, used in the treatment of Gout. Complementary in vivo cellular assays explain the molecular basis for α-KG driven drug elimination and the allosteric regulation of organic anion transport in the kidney by chloride.
External linksNat Struct Mol Biol / PubMed:37482561 / PubMed Central
MethodsEM (single particle)
Resolution3.43 - 3.94 Å
Structure data

16269

8bvr

EMDB-16269, PDB-8bvr:
Cryo-EM structure of rat SLC22A6 in the apo state
Method: EM (single particle) / Resolution: 3.52 Å

16270

8bvs

EMDB-16270, PDB-8bvs:
Cryo-EM structure of rat SLC22A6 bound to tenofovir
Method: EM (single particle) / Resolution: 3.61 Å

16271

8bvt

EMDB-16271, PDB-8bvt:
Cryo-EM structure of rat SLC22A6 bound to probenecid
Method: EM (single particle) / Resolution: 3.94 Å

16280

8bw7

EMDB-16280, PDB-8bw7:
Cryo-EM structure of rat SLC22A6 bound to alpha-ketoglutaric acid
Method: EM (single particle) / Resolution: 3.53 Å

16977

8omu

EMDB-16977, PDB-8omu:
Cryo-EM structure of rat SLC22A6 bound to alpha-ketoglutaric acid in a low occupancy state
Method: EM (single particle) / Resolution: 3.43 Å

Chemicals

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-TFO:
[2-(6-AMINO-9H-PURIN-9-YL)-1-METHYLETHOXY]METHYLPHOSPHONIC ACID / medication, antiretroviral*YM / Tenofovir disoproxil

ChemComp-CL:
Unknown entry / Chloride

ChemComp-RTO:
4-(dipropylsulfamoyl)benzoic acid

ChemComp-AKG:
2-OXOGLUTARIC ACID / Α-Ketoglutaric acid

Source
  • rattus norvegicus (Norway rat)
  • synthetic construct (others)
KeywordsMEMBRANE PROTEIN / transporter / METAL TRANSPORT

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