Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Structure and mechanism of the type I-G CRISPR effector.
Journal, issue, pages	Nucleic Acids Res, Vol. 50, Issue 19, Page 11214-11228, Year 2022
Publish date	Oct 28, 2022
Authors	Qilin Shangguan / Shirley Graham / Ramasubramanian Sundaramoorthy / Malcolm F White /
PubMed Abstract	Type I CRISPR systems are the most common CRISPR type found in bacteria. They use a multisubunit effector, guided by crRNA, to detect and bind dsDNA targets, forming an R-loop and recruiting the Cas3 ...Type I CRISPR systems are the most common CRISPR type found in bacteria. They use a multisubunit effector, guided by crRNA, to detect and bind dsDNA targets, forming an R-loop and recruiting the Cas3 enzyme to facilitate target DNA destruction, thus providing immunity against mobile genetic elements. Subtypes have been classified into families A-G, with type I-G being the least well understood. Here, we report the composition, structure and function of the type I-G Cascade CRISPR effector from Thioalkalivibrio sulfidiphilus, revealing key new molecular details. The unique Csb2 subunit processes pre-crRNA, remaining bound to the 3' end of the mature crRNA, and seven Cas7 subunits form the backbone of the effector. Cas3 associates stably with the effector complex via the Cas8g subunit and is important for target DNA recognition. Structural analysis by cryo-Electron Microscopy reveals a strikingly curved backbone conformation with Cas8g spanning the belly of the structure. These biochemical and structural insights shed new light on the diversity of type I systems and open the way to applications in genome engineering.
External links	Nucleic Acids Res / PubMed:36305833 / PubMed Central
Methods	EM (single particle)
Resolution	3.2 - 8.0 Å
Structure data	15540 8ane EMDB-15540, PDB-8ane: Structure of the type I-G CRISPR effector Method: EM (single particle) / Resolution: 3.2 Å 15820 8b2x EMDB-15820, PDB-8b2x: Structure of the type I-G CRISPR effector Method: EM (single particle) / Resolution: 8.0 Å
Source	thioalkalivibrio sulfidiphilus hl-ebgr7 (bacteria) synthetic construct (others)
Keywords	IMMUNE SYSTEM / CRISPR effector / GENE REGULATION / CRISPR / Effector