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TitleArchitecture of the MKK6-p38α complex defines the basis of MAPK specificity and activation.
Journal, issue, pagesScience, Vol. 381, Issue 6663, Page 1217-1225, Year 2023
Publish dateSep 15, 2023
AuthorsPauline Juyoux / Ioannis Galdadas / Dorothea Gobbo / Jill von Velsen / Martin Pelosse / Mark Tully / Oscar Vadas / Francesco Luigi Gervasio / Erika Pellegrini / Matthew W Bowler /
PubMed AbstractThe mitogen-activated protein kinase (MAPK) p38α is a central component of signaling in inflammation and the immune response and is, therefore, an important drug target. Little is known about the ...The mitogen-activated protein kinase (MAPK) p38α is a central component of signaling in inflammation and the immune response and is, therefore, an important drug target. Little is known about the molecular mechanism of its activation by double phosphorylation from MAPK kinases (MAP2Ks), because of the challenge of trapping a transient and dynamic heterokinase complex. We applied a multidisciplinary approach to generate a structural model of p38α in complex with its MAP2K, MKK6, and to understand the activation mechanism. Integrating cryo-electron microscopy with molecular dynamics simulations, hydrogen-deuterium exchange mass spectrometry, and experiments in cells, we demonstrate a dynamic, multistep phosphorylation mechanism, identify catalytically relevant interactions, and show that MAP2K-disordered amino termini determine pathway specificity. Our work captures a fundamental step of cell signaling: a kinase phosphorylating its downstream target kinase.
External linksScience / PubMed:37708276 / PubMed Central
MethodsEM (single particle) / X-ray diffraction
Resolution2.422 - 4.0 Å
Structure data

15233

8a8m

EMDB-15233, PDB-8a8m:
Structure of the MAPK p38alpha in complex with its activating MAP2K MKK6
Method: EM (single particle) / Resolution: 4.0 Å

PDB-5etc:
Structure of inactive MAPK14 with ordered Activation Loop
Method: X-RAY DIFFRACTION / Resolution: 2.422 Å

PDB-5eti:
Structure of dead kinase MAPK14
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-AP2:
PHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER

ChemComp-MG:
Unknown entry

Source
  • homo sapiens (human)
KeywordsTRANSFERASE / MAPK14 / inactive kinase / activation loop / MAPK / dead kinase / p38 / IMMUNE SYSTEM / Kinase / Signalling / MAP kinase / phosphoryl transfer

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